摘要： 采用UF-1型工业平板式超滤机对鸡新城疫病毒（NDV）及法氏囊病毒（IBDV）进行了初步的浓缩试验。该设备浓缩工艺简单、效率高，浓缩液HA效价与未浓缩液比较按浓缩比率相应上升。用浓缩液与未浓缩液制成灭活疫苗免疫鸡，均可获得100%抵抗ND及IBD的保护力，以浓缩苗免疫的NDHI抗体及IBD中和抗体滴度明显高于未浓缩苗。试验表明，浓缩苗安全有效，并优于未浓缩苗。
关键词:
鸡新城疫病毒,
法氏囊病毒,
浓缩,
灭活疫苗
Abstract: Preliminary experiments of concentrating NDV and IBDV were carried out by using an industrial plate-type UF-1 model ultrafilter. The equipment was simple in operating process and high in efficency of concentrating viruses. The HA titers of the concentrated virus fluids were higher than those of the unconcentrated virus fluids, and were proportional to the increase of concentration. The inactivated oil-adjuvant vaccines were prepared by using concentrated virus fluids and unconcentrated virus fluids. All chickens treated with concentrated vaccins and unconcentrated vaccines could gain 100% protection against virulent NDV and IBDV challenges. Both NDV HI and IBDV VN antibody titers of chickens treated with concentrated vaccinse were much higher than those treated with unconcentrated vaccines. The experimental results indicated that the concentrated vaccine was safer and better than the unconcentrated vaccine.
Key words:
Newcastle disease virus,
Infectious bursal disease virus,
Concentration,
Inactivated vaccine
郑世兰, 姜北宇, 刘福致, 张淑平, 景小冬, 李林, 姚颖. 用平板式超滤机浓缩鸡新城疫病毒及法氏囊病毒试验效果观察[J]. 华北农学报, 1999, 14(2): 139-144. doi: 10.3321/j.issn:1000-7091.1999.02.028.
Zheng Shilan, Jiang Beiyu, Liu Fuzhi, Zhang Shuping, Jing Xiaodong, Li Lin, Yao Ying. Experiments of Concentrating NDV and IBDV by Using Plate-type Ultrafilter[J]. ACTA AGRICULTURAE BOREALI-SINICA, 1999, 14(2): 139-144. doi: 10.3321/j.issn:1000-7091.1999.02.028.