摘要： 应用3批鸡传染性鼻炎二价油乳剂灭活疫苗进行了安全性和近期免疫效力检验，安全性试验采用15只鸡进行，胸肌注射疫苗4~5mL/只后，逐日观察，结果鸡只食欲、精神未见异常，2周后观察，注射部位未发现明显异常和病变。用该疫苗免疫（0.5mL/只）后的鸡只对A型高致病力菌株的攻击保护率可达100%，对C型高致病力菌株的攻击保护率为93.3%。采用B-ELISA和SPA对鸡群的抗体水平的评估表明该疫苗可激发产生较高的抗体水平。
关键词:
鸡传染性鼻炎,
二价油乳剂灭活疫苗,
安全性,
免疫效力
Abstract: Three batches of bivalent inactivated oil adjuvant vaccine to avian infectious coryza were produced and the safety and efficacy of them were also evaluated in this study.15 chickens were injected intramuscularly with 4-5 mL vaccine,there was no significant sign to be observed,and no lesions on the injecting spot after 2 weeks.The vaccine could achieve a protective rate about 100% against challenge ofhigh virulent strain of serovar A(Hp8)and 93.3% against challenge of serovar C(H18).The antibodies were also tested in this st udy by and SPA,and the results showed that the vaccine could stimulatehigh leve ls of antibody tohpg.All the results demonstrated the good safty and efficacy of the vaccine,and it could play a big role in the prevention of infectious coryza in China.
Key words:
Bivalent inactivated oil adjuvant vaccine,
Infecti ous coryza,
Safety,
Efficacy
中图分类号:
苗得园, 张培君, 龚玉梅, 冯文达, 冯杉. 鸡传染性鼻炎二价油乳剂灭活疫苗的近期免疫效力检验[J]. 华北农学报, 2000, 15(2): 138-142. doi: 10.3321/j.issn:1000-7091.2000.02.029.
MIAO Deyuan, ZHANG Peijun, GONG Yumei, FENG Wenda, FENG Shan. Tests of Efficacy of Bivalent Inactivated Oil Adjuvant Vaccine to Avian Infectious Coryza[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2000, 15(2): 138-142. doi: 10.3321/j.issn:1000-7091.2000.02.029.