摘要： 应用阻断ELISA（B-ELISA）、血凝抑制试验（HI）和血清平板凝集试验（SPA）方法，检测了鸡群用鸡传染性鼻炎A、C二价油乳剂灭活疫苗免疫后0~390d的血清阻断ELISA、HI和平板凝集抗体消长情况，并绘制了相应抗体曲线。结果表明，鸡体按规定免疫程序免疫后，A型B-ELISA抗体和HI抗体在测定期内一直保持比较高的水平，C型B-ELISA抗体首免0~180d、SPA抗体在首免后0~60d达到高峰，此后抗体缓慢下降，但各种抗体至少可持续390d以上。结果进一步显示了二价苗良好的免疫原性以及B-ELISA和SPA较好的敏感性，为二价苗、各种诊断方法或其试剂盒产品的进一步应用提供了参考。
关键词:
阻断ELISA,
血凝抑制试验,
血清平板凝集试验,
鸡传染性鼻炎二价油乳剂灭活苗,
抗体曲线
Abstract: The antibodies in sera from chickens vaccinated with bivalent vaccine of Infectious Coryza were measured by B ELISA,HI and SPA,and the antibody curves were also protracted.The results showed that the B ELISA antibody to serovar A and HI antibodies remained a high level post vaccination;the C serovar B ELISA antibody and SPA antibodies reached their vertex at 50-180 days and 50-60 days,respectively,and after that the level of the antibodies decreased slowly.All the antibodies could be detected more than 390 days post vaccination.These results also demonstrated the good sensitivity of the B ELISA and SPA,and gave a reference for the further application of the bivalent vaccine,all the diagnostic methods and correlative products.
Key words:
Blocking ELISA,
HI,
SPA,
Bivalent Inactivated Oil Adjuvant Vaccine to Infectious Coryza,
Antibody curve
中图分类号:
苗得园, 张培君, 龚玉梅, 苗德荣. 鸡传染性鼻炎二价油苗免疫鸡群抗体动态研究[J]. 华北农学报, 2001, 16(3): 135-139. doi: 10.3321/j.issn:1000-7091.2001.03.027.
MIAO Deyuan,ZHANG Peijun, GONG Yumei, MIAO Derong. Studies on Dynamics of Antibodies to Haemophilus Paragallinarum in Sera from Chickens Vaccinated with Bivalent Inactivated Oil Adjuvant Vaccine to Infectious Coryza[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2001, 16(3): 135-139. doi: 10.3321/j.issn:1000-7091.2001.03.027.