磷酸化姬松茸多糖制备、安全性及抑菌性研究

doi:10.7668/hbnxb.20191854

华北农学报 ›› 2020, Vol. 35 ›› Issue (S1): 371-377. doi: 10.7668/hbnxb.20191854

• 资源环境·植物保护 • 上一篇下一篇

磷酸化姬松茸多糖制备、安全性及抑菌性研究

路垚¹, 杨琳燕², 朱清杰³, 马吉飞², 李桂霞⁴, 胡德宝², 李瑞忠⁴, 常瑛军⁵

1. 天津市农业科学院农业资源与环境研究所, 天津 300192;
2. 天津农学院动物科学与动物医学学院, 天津 300384;
3. 河北省农村社会事业发展中心, 河北石家庄 050000;
4. 石家庄飞龙饲料有限公司, 河北石家庄 050073;
5. 河北一兽药业有限公司, 河北石家庄 050070

收稿日期:2020-10-09 出版日期:2020-12-28
通讯作者: 马吉飞(1963-),男,河北藁城人,教授,博士,主要从事分子免疫病理学研究。
作者简介:路垚(1989-),女,山东临沂人,研究实习员,硕士,主要从事农业微生物研究。
基金资助:
河北省重点研发计划项目（19227523D）；石家庄市科学技术研究与发展计划（196500407A）

Research on Preparation, Safety and Antibacterial Activity of Phosphorylated Agaricus blazei Murrill Polysaccharide

LU Yao¹, YANG Linyan², ZHU Qingjie³, MA Jifei², LI Guixia⁴, HU Debao², LI Ruizhong⁴, CHANG Yingjun⁵

1. Institute of Agricultural Resources and Environment Sciences and Animal Medicine, Tianjin Academy of Agricultural Sciences, Tianjin 300192, China;
2. College of Animal Science, Tianjin Agricultural University, Tianjin 300384, China;
3. Rural Social Development Center of Hebei Province, Shijiazhuang 050000, China;
4. Shijiazhuang Feilong Feed Co. LTD, Shijiazhuang 050073, China;
5. Hebei Yishou Pharmaceutical Co. LTD, Shijiazhuang 050070, China

Received:2020-10-09 Published:2020-12-28

摘要/Abstract

摘要： 为了研究磷酸化姬松茸多糖的制备工艺、安全性及抑菌效果，通过一系列试验进行研究。在磷酸化姬松茸多糖的制备工艺试验中，采用单因素和正交试验研究试剂配比、物料比、时间、温度和pH值对姬松茸多糖磷酸化的影响；在磷酸化姬松茸多糖的安全性评价试验中，利用小鼠急性毒性试验，验证其安全性；在磷酸化姬松茸多糖的抑菌试验中，用磷酸化姬松茸多糖和姬松茸多糖进行对比，验证两者对链球菌、大肠杆菌、双球菌、沙门氏菌的抑菌效果。磷酸化姬松茸多糖制备工艺的研究结果：STPP与STMP比例6：1，物料比1：3，时间5.5 h，温度95℃，pH值9，此时为最佳反应条件，磷酸根接枝量为9.14%；磷酸化姬松茸多糖的急性毒性试验结果：小鼠各项指标均正常，具备安全性；磷酸化姬松茸多糖的抑菌试验结果：经过磷酸化和未经磷酸化的姬松茸多糖对沙门氏菌和大肠杆菌均有抑菌作用，且磷酸化效果更好。通过上述三部分试验，研究出了磷酸根接枝量较高的磷酸化方法，证明了磷酸化姬松茸多糖的安全性，并具有较好抑菌效果，具有进一步研究价值。

关键词: 磷酸化姬松茸多糖, 安全性评价, 急性毒性试验, 抑菌, 单因素试验

Abstract: In order to explore the preparation technology,safety and bacteriostatic effect of phosphorylated Agaricus blazei Murrill polysaccharide,a series of experiments were carried out.Single factor and orthogonal design method were used to observe the influences from phosphorylation reagent ratio,material ratio,time,temperature and pH value on the phosphate-group grafting amount of ABMP.In the safety evaluation test of phosphorylated ABMP,the acute toxicity test in mice was used to evaluate the safety.In the antibacterial test of the phosphoylated ABMP,the phosphoylated ABMP and ABMP were compared to verify the bacteriostatic effect on Streptococcus,Escherichia coli,Diplococcus and Salmonella bacteria.Results showed that the STPP/STMP ratio was 6:1,material ratio was 1:3,time was 5.5 h,temperature was 95℃,and pH value was 9,which was the optimal reaction condition.And the phosphate-group grafting amount at this moment was 9.14%.The acute toxicity test of phosphorylated ABMP showed that all indexes of mice were normal and safe.The results of antibacterial tests showed that the phosphoylated ABMP and ABMP had bacteriostatic effect on Salmonella bacteria and Escherichia coli,and the phosphorylated ABMP was better.Through the above three part tests,we obtained a good phosphorylation method and proved the safety of phosphorylated ABMP,and proved that the phosphorylated ABMP has a good bacteriostatic effect,which had further research value.

Key words: Phosphorylated Agaricus blazei Murrill Polysaccharide, Safety evaluation, Acute toxicity test, Antibacterial, Single factor test

中图分类号:

S432.4

路垚, 杨琳燕, 朱清杰, 马吉飞, 李桂霞, 胡德宝, 李瑞忠, 常瑛军. 磷酸化姬松茸多糖制备、安全性及抑菌性研究[J]. 华北农学报, 2020, 35(S1): 371-377. doi: 10.7668/hbnxb.20191854.

LU Yao, YANG Linyan, ZHU Qingjie, MA Jifei, LI Guixia, HU Debao, LI Ruizhong, CHANG Yingjun. Research on Preparation, Safety and Antibacterial Activity of Phosphorylated Agaricus blazei Murrill Polysaccharide[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(S1): 371-377. doi: 10.7668/hbnxb.20191854.

http://www.hbnxb.net/CN/Y2020/V35/IS1/371

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