摘要： 采用NDLasota株为种毒，福尔马林为灭活剂，7号白油为佐剂，吐温80、司本80为乳化剂，硫柳汞为防腐剂，高压匀浆泵为乳化工具，研制出ND油性剂灭活疫苗。其剂型为双相型（W/O/W），呈稀薄乳状液，粒度为1μm左右，粘度≤1s，通针性好。4～8℃有效保存期1年。效力≥70PD50，最早产生免疫时间为12天（100%保护），生产中常用免疫剂量为2月龄内每只0.5ml，2月龄以上每只1.0ml皮下注射，对各日龄鸡安全无副作用。与ND活毒疫苗同时免疫可使各日龄鸡产生高效价的HI抗体，雏鸡免疫期为2.5～3个月，成年鸡为12个月。此苗可有效地预防强毒力嗜内脏型ND及“非典型”ND.

关键词: 新城疫（ND）, 灭活疫苗, 油佐剂

Abstract: Inactivated oil emulsion vaccine against ND was studied and prepared successfully,by using NDV Lasota strain as seed virus,formalin as inactivating agent,No 7 white mineral oil as adjuvant, Tween-80 and span-80 as emulsifiers, merthiolate as preservative. This oil-emulsion vaccine, of a double emulsion type (W/O/W), had particles about 1μm in diameter. The vaccine was thin emulsion with the viscosity≤1 second,it was easy to inject. Under 4~8℃,the vaccine can be preserved for over one year. Potency tests indicated the dosage was ≥70PD₅₀ The earliest immunity was produced at 12 days after inoculation (100% prortection). Practically, the preferred dosage was 0. 5ml per bird for birds within 2 months of age, and 1. 0ml per bird for the birds older than 2 months of age. The vaccine was safe for chickens of any ages, and had no adverse effects. When combined with live ND vaccines, it could induce high HI antibody for chicks of any ages. Immune periods lasted 2. 5 to 3 months for chickens and more than 6 months for adult birds. The viscerotropic velogenic ND and atypical ND can be prevented effectively by using this vaccine.

Key words: Newcastle Disease (ND), Inactivated vaccine, Oil Emulsion

中图分类号: 

• S852.5