摘要： 以IBH₁₂₀鸡胚毒适应成纤维细胞（CEF）单层，培养出IBH₁₂₀细胞弱毒株。电镜观察，可见典型的冠状病毒粒子。IBH₁₂₀细胞弱毒株经细菌、霉菌、支原体、鸡白血病病毒检测均为阴性，且具有IB病毒特异性。CEF单层接毒后48h左右病毒增殖达到高峰。随着细胞传代次数的增加，C₄~C₁₀代H₁₂₀株细胞毒的毒价（TCID₅₀）逐步增高而趋于稳定，C₄~C₁₀代细胞毒的TCID₅₀稳定在10^7.35~8.0mL之间，而C₅~C₁₀代IBH₁₂₀细胞毒的EID₅₀呈下降趋势，变动范围在10^7.3~10^6.5mL。以10^3.5TCID₅₀/只和10^4.5TCID₅₀/只的剂量，C₄，C₈，C₁₀代毒分别滴鼻接种3日龄敏感鸡，免疫鸡的攻毒保护率为80%~100%，病毒分离回收率分别为20%和0。

关键词: 鸡传染性支气管炎H₁₂₀细胞弱毒株, 繁殖曲线, 免疫原性, 免疫保护率

Abstract: Cellular attenuated strain of infection bronchitis H₁₂₀ strain(IBH₁₂₀)was cultured successfully with virus which cultured on the chicken embryo.Atypical Coronary viral partical were observed by electric microscope.IBH₁₂₀ cellular attenuated strains were negative after examination bacterium,mildew,mycoplasma,chicken leukemia virus,moreover,they speciticity of IB virus.Virus proliferate could reach peak after 48 h.When CEF cells infected with IBH₁₂₀,TCID₅₀ of C₄-C₁₀ generation of H₁₂₀ strain cellular attenuated virus rose gradually and stabilized with increasing generation.TCID₅₀ of C₄-C₁₀ generations were between 10^7.35 and 10^8.0.but EID₅₀ of C₅-C₁₀generation were degressive trend.The variational range was between 10^7.3 and 10^6.5.Theimmunogenicity of IBH₁₂₀ cellular attenuated virus was checked.The result demonstrated that three days old chickens were inoculated in nose with 10^3.5 TCID₅₀ and 10^4.5 TCID₅₀ of C₄,h,C₁₀ generation virus in each chicken respectively,their protection rate was between 80% and 100%.The virus separation recovery rate was 20% and 0 respectivelly.

Key words: Cellular attenuated strain of IBH₁₂₀, Breeding curve, Immunogenicity, Immune protection rate

中图分类号: 

• S436.612