摘要： 结合形态与分子生物学特征对从河北省分离的40个异小杆类昆虫病原线虫种群进行鉴定，其主要形态特征（4个典型种群平均值）：侵染性幼虫体长=550（520~610）μm，体宽=22.2（20~24）μm，尾长=85.7（80~88）μm，E%=112.5%（105%~119%）；雄虫交合刺长度=36.8（33.75~39.0）μm，引带长度=17.9（16.3~20.0）μm，GS%=0.49（0.41~0.59），SW=1.25（1.15~1.43），与嗜菌异小杆线虫Heterorhabditis bacteriophora形态特征相近。经对其中二个典型种群的ITS-rDNA测序，其ITS1-rDNA序列与已知种类H.bacteriophora的ITS1-rDNA序列相差小于1.2%，和H.argentinensis的差异小于1.5%，而与其他种类的差异则高达18.7%以上。综合其形态与分子特征，确定河北省的异小杆类线虫为嗜菌异小杆线虫。

关键词: 昆虫病原线虫, 嗜菌异小杆线虫, 种类鉴定, 生物防治

Abstract: 40 Heterorhabditis spp.populations isolated from Hebei province were identified based on the morphological and molecular characters.The main morphometrical characteristics based on the average of four typical populations are as followed:infective juvenile length=550(520-610)μm,tail length=85.7(80-88)μm,body width =22.2(20-24)μm,E%=112.5%(105%-119%);Male spicule =36.8(33.7539.0)μm,gubernaculum length =17.9(16.3-20.0)μm,GS%=0.49(0.41-0.59),SW=1.25(1.15-1.43),they are mostly close to H.bacteriophora in morphology.The ITS-rDNA of two local populations was sequenced.The ITS1-rDNA sequence differences are less than 1.2% between local populations and H.bacteriophora,less than 1.5% between local populations and H.argentinensis,but over 18.7% between local populations and other known species.It is proved that the Heterorhabditis spp.populations isolated from Hebei province are H.bacteriophora based on morphological and molecular characters.

Key words: Entomopathogenic nematodes, Heterorhabditis bacteriophora, Ident ification, Biological control

中图分类号: 

• S435.622