摘要： 用10—30KR剂量⁶⁰CO—γ射线处理小麦中引15、St和水稻沪B干种子、幼苗生长期胚根、子叶发育受到明显抑制.对幼苗生长的影响后期（萌发5天后）比早期（开始萌发至萌发第5天）明显,芽鞘的发育对γ射线不敏感.过氧化物酶同工酶的分析结果表明,发育受抑的小麦St、水稻沪B胚根中各增加1—2条负极酶带;发育受抑的小麦中引15、St、水稻沪B子叶中,过氧化物酶同工酶没有产生变化,而从外部形态特征上看没有变化的中引15芽鞘中比对照多2条正极带.上述结果表明,经r射线照射后,植物外部形态特征的变化与内部过氧化物酶同工酶的变化并不完全相关.

关键词: 过氧化物酶同工酶, 负极向, 小麦, 水稻

Abstract: The seed of wheat (Triticum asstivum) and rice (Oryza sative) had been irradiated with different doses (0,10KR, 20KR, 30KR) of 60Co ?-ray before sowing. The experiment indicated that the growth of radicles and leafs were inhibited by irradiation, but, no effect of 60Co ?-ray on coleo-ptile growth were showed at seedling stage. The germination of seed both in wheat and rice were not affected by irradiation. The experiment also showed that the inhibition effect on growth is much more in early stage of seedling than that in old stage.The results of analysis by peroxidase isozymes were illustrated in Fig4and Fig5 (a, b). There were differences in the zymograme patterns of the peroxidase isozymes of radicle between the irradiated and non-irradited seeds of wheat and rice. There were two cathode bands in zymograme of radicle with ?-ray more than of non-irradiated.However, there was no obvious difference on Peroxidase leaf. According to above, it seems there are no relative between the changes of morphogenesis structure and peroxidase isozymes.

Key words: Peroxidase isozymes, Cathode, Wheat, Rice