Research on Antioxidant Activity of Red-fresh Apple Fruit

doi:10.7668/hbnxb.20195115

Abstract

Abstract:

Malus sieversii M.Roem.exhibits exceptionally high genetic diversity,and the red-fresh apple is considered a variety within this species.It investigated the antioxidant activity of red-fresh apple fruits during ripening and postharvest storage,as well as the changing trends in antioxidant enzyme activities,aiming to provide a reference for the exploitation and utilization of M.sieversii and the selection and breeding of functional apples.Using Huashuo as a control,the contents of total phenols,total flavonoids,and free radical scavenging capacity in four red-fresh apple varieties were studied,along with the activity trends of related antioxidant enzymes during storage.The results showed that the total phenol and total flavonoid contents in the four red-fresh apple resource fruits ranged from 1.42-3.12 mg/g and 19.36-64.81 μg/g,respectively,which were significantly higher than those of the control Huashuo,with Ke showing extremely significant differences.The free radical scavenging capacity was higher than that of the control group,but the difference was not statistically significant.A significant positive correlation was observed between total phenols and total flavonoids,as well as between total phenols and 2,2'-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)diammonium salt(ABTS),and between total flavonoids and ABTS.Throughout the storage period,the activities of polyphenol oxidase,superoxide dismutase,and catalase in Ke were higher than those in the other varieties.Ke can be used as a functional apple parent material for breeding offspring with high antioxidant capacity,thereby facilitating the development and utilization of germplasm resources in M.sieversii.

Key words: Red-fresh apple, Total phenols, Total flavonoids, Antioxidant enzyme oxidase

CLC Number:

S661.1

RAN Bian, ZHANG Shengjun, TANG Shimin, TIAN Fang, DONG Shilei, ZHU Ling, ZHANG Xuechao. Research on Antioxidant Activity of Red-fresh Apple Fruit[J]. Acta Agriculturae Boreali-Sinica, 2025, 40(S1): 138-143. doi: 10.7668/hbnxb.20195115.

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References 35

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资源 Varieties	总酚含量/(mg/g) Total phenolic content	总黄酮含量/(μg/g) Total flavonoid content	DPPH/ (μg/g)	FRAP/ (mmol/(L·g))	ABTS/ (mmol/(L·g))
桑Sang	1.46±0.06Bb	24.23±0.77Bb	386.81±3.83ABa	1.74±0.09Aa	8.45±0.15Aab
中Zhong	1.42±0.05Bb	21.88±0.77Bb	390.41±2.58Aa	1.69±0.01ABa	7.66±0.07Abc
克Ke	3.12±0.11Aa	64.81±3.58Aa	396.22±12.19Aa	1.75±0.03Aa	8.65±0.47Aa
夏Xia	1.42±0.04Bb	19.36±1.55Bbc	396.78±10.72Aa	1.83±0.04Aa	7.71±0.33Abc
华硕Huashuo	1.05±0.03Cc	14.60±2.07Bc	376.76±4.21Aa	1.27±0.04Bb	7.44±0.16Ac

资源 Varieties	总酚含量/(mg/g) Total phenolic content	总黄酮含量/(μg/g) Total flavonoid content	DPPH/ (μg/g)	FRAP/ (mmol/(L·g))	ABTS/ (mmol/(L·g))
桑Sang	1.46±0.06Bb	24.23±0.77Bb	386.81±3.83ABa	1.74±0.09Aa	8.45±0.15Aab
中Zhong	1.42±0.05Bb	21.88±0.77Bb	390.41±2.58Aa	1.69±0.01ABa	7.66±0.07Abc
克Ke	3.12±0.11Aa	64.81±3.58Aa	396.22±12.19Aa	1.75±0.03Aa	8.65±0.47Aa
夏Xia	1.42±0.04Bb	19.36±1.55Bbc	396.78±10.72Aa	1.83±0.04Aa	7.71±0.33Abc
华硕Huashuo	1.05±0.03Cc	14.60±2.07Bc	376.76±4.21Aa	1.27±0.04Bb	7.44±0.16Ac

项目 Item	总酚 Total phenolic	总黄酮 Total flavonoid	DPPH	FRAP	ABTS
总酚Total phenolic	1.000	0.985^**	0.308	0.407	0.646^**
总黄酮Total flavonoid		1.000	0.282	0.351	0.685^**
DPPH			1.000	0.449	0.569
FRAP				1.000	0.494
ABTS					1.000

项目 Item	总酚 Total phenolic	总黄酮 Total flavonoid	DPPH	FRAP	ABTS
总酚Total phenolic	1.000	0.985^**	0.308	0.407	0.646^**
总黄酮Total flavonoid		1.000	0.282	0.351	0.685^**
DPPH			1.000	0.449	0.569
FRAP				1.000	0.494
ABTS					1.000

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