Effects of Exogenous Melatonin on Antioxidant System of Potato Seedlings under Low Temperature Stress

doi:10.7668/hbnxb.20192429

Abstract

Abstract:

In order to explore the effects of different concentrations of exogenous melatonin on the growth of potato seedlings under low temperature stress after cold acclimation,using Yunnan main potato variety Hezuo 88 as material,the effects of different concentrations of exogenous melatonin(50,100,150 μmol/L)on the growth and active oxygen metabolism system of potato seedlings under low temperature stress(4 ℃ 14 d ,-2 ℃ 12 h)were studied.The purpose of this study was to explore the feasibility of exogenous MT in alleviating the injury of potato seedlings caused by low temperature stress.The results showed that compared with potato seedlings under low temperature stress,the survival rate of seedlings treated with 50 μmol/L MT increased by 38.89 percentage point,and the plant height and stem diameter increased by 2.92% and 1.86%,the contents of SS,SP and Pro increased by 12.66%,13.80% and 1.96%,the activities of POD and CAT increased by 29.24% and 351.62%.The plant height and stem diameter of seedlings treated with 100 μmol/L MT increased by 16.97% and 2.82%,the contents of MDA and H₂O₂ decreased by 19.35% and 0.48%,the activities of POD and CAT increased by 55.44% and 213.56%.The survival rate of seedlings treated with 150 μmol/L MT increased by 27.78 percentage point,the plant height and stem diameter increased by 5.62% and 13.20%,the content of MDA decreased by 9.45%,the content of Pro increased by 38.70%,SOD,POD and CAT activity increased by 52.61%,25.25% and 300.94%,and the content of AsA and GSH increased by 3.48% and 3.97%.The results showed that exogenous MT could promote the growth of potato seedlings,reduce the content of MDA and H₂O₂ in leaves,promote the accumulation of SS,SP and Pro,increase the activities of SOD,POD and CAT,and increase the content of AsA and GSH.Thus,it can alleviate the damage of low temperature stress to potato seedlings,and then improve the ability of potato seedlings to resist low temperature stress.In summary,50 μmol/L MT treatment could better improve the stress resistance of potato seedlings under low temperature stress.

Key words: Potato, Low temperature stress, Exogenous melatonin, Antioxidant system

HE Qiulan, ZHANG Hang, WANG Zhengwei, WAN Liqiang, HAI Meirong. Effects of Exogenous Melatonin on Antioxidant System of Potato Seedlings under Low Temperature Stress[J]. Acta Agriculturae Boreali-Sinica, 2022, 37(1): 103-111. doi: 10.7668/hbnxb.20192429.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.7668/hbnxb.20192429

http://www.hbnxb.net/EN/Y2022/V37/I1/103

Figures/Tables 14

Fig.1 Effect of exogenous melatonin on potato phenotype under low temperature stress

Fig.2 Effect of exogenous melatonin on potato survival rate under low temperature stress
WT.Normal temperature control;LT.Low temperature control;LT+50MT.Addition 50 μmol/L melatonin under low temperature stress;LT+100MT.Addition 100 μmol/L melatonin under low temperature stress;LT+150MT. Addition 150 μmol/L melatonin under low temperature stress. Different letters indicate significant difference among treatments(P<0.05).The same as Fig.3—14.

Fig.3 Effects of exogenous melatonin on plant height of potato under low temperature stress

Fig.4 Effects of exogenous melatonin on stem diameter of potato under low temperature stress

Fig.5 Effects of exogenous melatonin on H₂O₂ content in leaves of potato under low temperature stress

Fig.6 Effects of exogenous melatonin on MDA content in leaves of potato under low temperature stress

Fig.7 Effects of exogenous melatonin on SS content in leaves of potato under low temperature stress

Fig.8 Effects of exogenous melatonin on SP content in leaves of potato under low temperature stress

Fig.9 Effects of exogenous melatonin on Pro content in leaves of potato under low temperature stress

Fig.10 Effect of exogenous melatonin on SOD activity in leaves of potato under low temperature stress

Fig.11 Effect of exogenous melatonin on POD activity in leaves of potato under low temperature stress

Fig.12 Effect of exogenous melatonin on CAT activity in leaves of potato under low temperature stress

Fig.13 Effects of exogenous melatonin on AsA content in leaves of potato under low temperature stress

Fig.14 Effects of exogenous melatonin on GSH content in leaves of potato under low temperature stress

References 35

[1]	徐建飞,金黎平.马铃薯遗传育种研究: 现状与展望[J].中国农业科学,2017,50(6):990-1015.doi:10.3864/j.issn.0578-1752.2017.06.003.
	Xu J F,Jin L P.Advances and perspectives in research of potato genetics and breeding[J].Scientia Agricultura Sinica,2017,50(6):990-1015.
[2]	杨雅伦,郭燕枝,孙君茂.我国马铃薯产业发展现状及未来展望[J].中国农业科技导报,2017,19(1):29-36.doi:10.13304/j.nykjdb.2016.202.
	Yang Y L,Guo Y Z,Sun J M.Present status and future prospect for potato industry in China[J].Journal of Agricultural Science and Technology,2017,19(1):29-36.
[3]	孙根紧,王滨玥,刘彦秀.中国马铃薯种植区域比较优势的时空演进[J].西昌学院学报(自然科学版),2021,35(1):1-7,11.doi:10.16104/j.issn.1673-1891.2021.01.001.
	Sun G J,Wang B Y,Liu Y X.Research on spatial-temporal evolution of comparative advantages of potato planting regions in China[J].Journal of Xichang University(Natural Science Edition),2021,35(1):1-7,11.
[4]	郝若诗.云南省马铃薯绿色生产技术采用行为与效果研究[D].北京:中国农业科学院,2020.doi:10.27630/d.cnki.gznky.2020.000797.
	Hao R S.Study on the adoption behavior and effect of green potato production technology in Yunnan Province[D].Beijing:Chinese Academy of Agricultural Sciences,2020.
[5]	桑月秋,杨琼芬,刘彦和,卢丽丽,李文娟,周俊,谢开云,隋启君.云南省马铃薯种植区域分布和周年生产[J].西南农业学报,2014,27(3):1003-1008.doi:10.16213/j.cnki.scjas.2014.03.039.
	Sang Y Q,Yang Q F,Liu Y H,Lu L L,Li W J,Zhou J,Xie K Y,Sui Q J.Cultivated regional distribution and year-round production of potato in Yunnan Province[J].Southwest China Journal of Agricultural Sciences,2014,27(3):1003-1008.
[6]	李祎君,王春乙,赵蓓,刘文军.气候变化对中国农业气象灾害与病虫害的影响[J].农业工程学报,2010,26(S1):263-271.
	Li Y J,Wang C Y,Zhao B,Liu W J.Effects of climate change on agricultural meteorological disaster and crop insects diseases[J].Transactions of the Chinese Society of Agricultural Engineering,2010,26(S1):263-271.
[7]	麻吉亮,孔维升,朱铁辉.农业灾害的特征、影响以及防灾减灾抗灾机制-基于文献综述视角[J].中国农业大学学报(社会科学版),2020,37(5):122-129.doi:10.13240/j.cnki.caujsse.2020.05.013.
	Ma J L,Kong W S,Zhu T H.Characteristics,impacts of agricultural disaster,and mechanism of disaster prevention,mitigation and response-from the perspective of literature review[J].Journal of China Agricultural University (Social Sciences),2020,37(5):122-129.
[8]	桑婧,郝璐.近30年中国主要农业气象灾害典型场时空格局分异特征[J].中国生态农业学报,2018,26(9):1302-1314.doi:10.13930/j.cnki.cjea.170697.
	Sang J,Hao L.Spatio-temporal patterns of typical agro-meteorological disasters in China in the past 30 years[J].Chinese Journal of Eco-Agriculture,2018,26(9):1302-1314.
[9]	王孟宇.云南农业气候资源的现状及综合利用对策[J].西南农业学报,2010,23(2):598-601.doi:10.16213/j.cnki.scjas.2010.02.002.
	Wang M Y.Current situation and comprehensive utilization countermeasure of Yunnan agriculture climate resource[J].Southwest China Journal of Agricultural Sciences,2010,23(2):598-601.
[10]	吴广霞,唐献龙,杨德光,席景会.植物低温胁迫生理研究进展[J].作物杂志,2008(3):17-19.doi:10.3969/j.issn.1001-7283.2008.03.005.
	Wu G X,Tang X L,Yang D G,Xi J H.Research progress on physiology of plant cold hardiness[J].Crops,2008(3):17-19.
[11]	徐呈祥.提高植物抗寒性的机理研究进展[J].生态学报,2012,32(24):7966-7980.doi:10.5846/stxb201106260945.
	Xu C X.Research progress on the mechanism of improving plant cold hardiness[J].Acta Ecologica Sinica,2012,32(24):7966-7980.
[12]	张珍. 马铃薯早熟防寒栽培技术[J].河南农业,2013(5):46.doi: 10.3969/j.issn.1006-950X.2013.05.034.
	Zhang Z.Cultivation techniques of early ripening and cold protection of potato[J].Henan Nongye,2013(5):46.
[13]	Song Q P,You L L,Liu Y,Zhang J,Yang X H.Endogenous accumulation of glycine betaine confers improved low temperature resistance on transplastomic potato plants[J].Functional Plant Biology,2020,47(12):1105-1116.doi:10.1071/fp20059.
[14]	孙忠富. 霜冻灾害与防御技术[M].北京: 中国农业科技出版社,2001.
	Sun Z F.Frost disaster and prevention technology[M].Beijing:China Agricultural Science and Technology Press,2001.
[15]	许娟,郑虚,闫海锋,唐秀桦,熊军,韦民政,覃维治,李韦柳.不同马铃薯品种苗期叶片对低温胁迫的生理响应[J].南方农业学报,2016,47(11):1837-1843.doi:10.3969/jissn.2095-1191.2016.11.1837.
	Xu J,Zheng X,Yan H F,Tang X H,Xiong J,Wei M Z,Qin W Z,Li W L.Physiological responses of different potato varieties to cold stress at seedling stage[J].Journal of Southern Agriculture,2016,47(11):1837-1843.
[16]	Kou S,Chen L,Tu W,Scossa F,Wang Y M,Liu J,Fernie A R,Song B T,Xie C H.The arginine decarboxylase gene ADC1,associated to the putrescine pathway,plays an important role in potato cold-acclimated freezing tolerance as revealed by transcriptome and metabolome analyses[J].The Plant Journal,2018,96(6):1283-1298.doi:10.1111/tpj.14126.
[17]	杨慧菊,郭华春.马铃薯不同品种抗寒性综合评价[J].分子植物育种,2017,15(2):716-724.doi:10.13271/j.mpb.015.000716.
	Yang H J,Guo H C.Comprehensive evalution of cold resistence of potato varieties[J].Molecular Plant Breeding,2017,15(2):716-724.
[18]	舒启琼,罗小波,李飞,罗充.马铃薯抗寒研究进展[J].植物生理学报,2021,57(6):1171-1178.doi:10.13592/j.cnki.ppj.2020.0545.
	Shu Q Q,Luo X B,Li F,Luo C.Research progress on cold resistance of potato[J].Plant Physiology Journal,2021,57(6):1171-1178.
[19]	秦玉芝,陈珏,邢铮,何长征,熊兴耀.低温逆境对马铃薯叶片光合作用的影响[J].湖南农业大学学报(自然科学版),2013,39(1):26-30.doi:10.3724/SP.J.1238.2013.00026.
	Qin Y Z,Chen J,Xing Z,He C Z,Xiong X Y.Effects of low temperature stress on photosynthesis in potato leaves[J].Journal of Hunan Agricultural University(Natural Sciences),2013,39(1):26-30.
[20]	Paredes S D,Korkmaz A,Manchester L C,Tan D X,Reiter R J.Phytomelatonin:a review[J].Journal of Experimental Botany,2009,60(1):57-69.doi:10.1093/jxb/ern284.
[21]	赵燕,王东华,赵曦阳.植物中褪黑素的研究进展[J].西北植物学报,2014,34(1):196-205.doi:10.7606/j.issn.1000-4025.2014.01.0196.
	Zhao Y,Wang D H,Zhao X Y.Recent advance on the melatonin in plant[J].Acta Botanica Boreali-Occidentalia Sinica,2014,34(1):196-205.
[22]	王蕊,杨小龙,须晖,李天来.高等植物褪黑素的合成和代谢研究进展[J].植物生理学报,2016,52(5):615-627.doi:10.13592/j.cnki.ppj.2016.0052.
	Wang R,Yang X L,Xu H,Li T L.Research progress of melatonin biosynthesis and metabolism in higher plants[J].Plant Physiology Journal,2016,52(5):615-627.
[23]	姜超强,祖朝龙.褪黑素与植物抗逆性研究进展[J].生物技术通报,2015,31(4):47-55.doi:10.13560/j.cnki.biotech.bull.1985.2015.03.013.
	Jiang C Q,Zu C L.Advances in melatonin and its roles in abiotic stress resistance in plants[J].Biotechnology Bulletin,2015,31(4):47-55.
[24]	包宇,罗庆熙,黄娟,池浩.外源褪黑素对低温胁迫下番茄幼苗生理指标的影响[J].西南师范大学学报(自然科学版),2013,38(10):57-61.doi:10.13718/j.cnki.xsxb.2013.10.019.
	Bao Y,Luo Q X,Huang J,Chi H.On effect of exogenous melatonin on physiological indexes of tomato seedling under low temperature stress[J].Journal of Southwest China Normal University(Natural Science Edition),2013,38(10):57-61.
[25]	Aghdam M S,Luo Z S,Jannatizadeh A,Sheikh-Assadi M,Sharafi Y,Farmani B,Fard J R,Razavi F.Employing exogenous melatonin applying confers chilling tolerance in tomato fruits by upregulating ZAT2/6/12 giving rise to promoting endogenous polyamines,proline,and nitric oxide accumulation by triggering arginine pathway activity[J].Food Chemistry,2019,275:549-556.doi:10.1016/j.foodchem.2018.09.157.
[26]	吴雪霞,朱宗文,张爱冬,许爽,姚静,查丁石.外源褪黑素对低温胁迫下茄子幼苗生长及其光合作用和抗氧化系统的影响[J].西北植物学报,2017,37(12):2427-2434.doi:10.7606/j.issn.1000-4025.2017.12.2427.
	Wu X X,Zhu Z W,Zhang A D,Xu S,Yao J,Zha D S.Effects of exogenous melatonin on the growth,photosynthesis and antioxidant system in eggplant(Solanum melongena L.)seedlings under low temperature stress[J].Acta Botanica Boreali-Occidentalia Sinica,2017,37(12):2427-2434.
[27]	王芳,王淇,赵曦阳.低温胁迫下植物的表型及生理响应机制研究进展[J].分子植物育种,2019,17(15):5144-5153.doi:10.13271/j.mpb.017.005144.
	Wang F,Wang Q,Zhao X Y.Research progress of phenotype and physiological response mechanism of plants under low temperature stress[J].Molecular Plant Breeding,2019,17(15):5144-5153.
[28]	尉欣荣,张智伟,周雨,刘鹏,姚宏斌,苗彦军,付娟娟.褪黑素对低温和干旱胁迫下多年生黑麦草幼苗生长和抗氧化系统的调节作用[J].草地学报,2020,28(5):1337-1345.doi:10.11733/j.issn.1007-0435.2020.05.019.
	Yu X R,Zhang Z W,Zhou Y,Liu P,Yao H B,Miao Y J,Fu J J.Effects of melatonin on growth and antioxidant system of perennial ryegrass seedlings under cold and drought stresses[J].Acta Agrestia Sinica,2020,28(5):1337-1345.
[29]	李欠敏,杨琳,段巧红,黄家保.外源褪黑素对低温胁迫下大白菜幼苗生长及生理特性的影响[J].山东农业大学学报(自然科学版),2021,52(2):182-186.doi:10.3969/j.issn.1000-2324.2021.02.004.
	Li Q M,Yang L,Duan Q H,Huang J B.Effects of exogenous melatonin on the growth and physiological characteristics of Chinese cabbage seedlings under chilling stress[J].Journal of Shandong Agricultural University(Natural Science Edition),2021,52(2):182-186.
[30]	唐懿,余雪娜,何娟,张恩泽,兰静宇,李焕秀.外源褪黑素对低温弱光胁迫下茄子幼苗生理特性的影响[J].湖南农业大学学报(自然科学版),2017,43(3):257-261.doi:10.13331/j.cnki.jhau.2017.03.006.
	Tang Y,Yu X N,He J,Zhang E Z,Lan J Y,Li H X.Effects of exogenous melatonin on physiological properties of eggplant(Solanum melongena L.)seedlings under the stress of low light and low temperature[J].Journal of Hunan Agricultural University(Natural Sciences),2017,43(3):257-261.
[31]	史中飞,梁娟红,张小花,成宏斌,郑晟,王娟,张腾国.外源褪黑素对低温胁迫下油菜幼苗抗寒性的影响[J].干旱地区农业研究,2019,37(4):163-170.doi:10.7606/j.issn.1000-7601.2019.04.22.
	Shi Z F,Liang J H,Zhang X H,Cheng H B,Zheng S,Wang J,Zhang T G.Effect of exogenous melatonin on cold resistance of Brassica rapa seedlings under low temperature stress[J].Agricultural Research in the Arid Areas,2019,37(4):163-170.
[32]	张嘉雯,卢绍浩,赵喆,赵铭钦.外源褪黑素对低温胁迫下烟草幼苗生理指标的影响[J].中国农业科技导报,2020,22(9):78-86.doi:10.13304/j.nykjdb.2019.0578.
	Zhang J W,Lu S H,Zhao Z,Zhao M Q.Influences of exogenous melatonin on physiological properties of tobacco seedlings under low temperature stress[J].Journal of Agricultural Science and Technology,2020,22(9):78-86.
[33]	赵小红,罗庆熙,饶玲.外源褪黑素在低温胁迫下对黄瓜幼苗抗冷性的影响[J].北方园艺,2017(14):55-59.doi:10.11937/bfyy.20164959.
	Zhao X H,Luo Q X,Rao L.Effects of exogenous melatonin on chilling tolerance of cucumber seedlings under low temperature stress[J].Northern Horticulture,2017(14):55-59.
[34]	Li Y L,Liu Y F,Zhang J G.Advances in the research on the AsA-GSH cycle in horticultural crops[J].Frontiers of Agriculture in China,2010,4(1):84-90.doi:10.1007/s11703-009-0089-8.
[35]	罗娅,汤浩茹,张勇.低温胁迫对草莓叶片SOD和AsA-GSH循环酶系统的影响[J].园艺学报,2007,34(6):1405-1410.doi:10.16420/j.issn.0513-353x.2007.06.010.
	Luo Y,Tang H R,Zhang Y.Effect of low temperature stress on activities of SOD and enzymes of ascorbate-glutathione cycle[J].Acta Horticulturae Sinica,2007,34(6):1405-1410.

Please choose a citation manager

Content to export