Identification of AQP7 Gene and the Expression and Location in Various Tissues of Jiulong Yak

doi:10.7668/hbnxb.20191828

Abstract

Abstract: The aim of this study was to identify the Aquaporin 7 gene and analyze the expression level and protein localization of AQP7 gene in different tissues of yak.The AQP7 gene of Jiulong yak was cloned by RT-PCR and analyzed by bioinformatics.RT-qPCR was used to detect the expression levels in 8 different tissues of yaks.Immunohistochemical staining method was used to analyze the expression and location of AQP7 protein in tissues.The results showed that the CDS sequence of AQP7 gene was 993 bp, which encoded 330 amino acids. Bioinformatics analysis found that AQP7 was a stable hydrophobic protein.The phylogenetic tree showed that AQP7 gene of Jiulong yak was closely related to cattle, and its homology was 99.7%. The expression of AQP7 gene was higher in Jiulong yak heart and muscle, which were significantly higher than that in kidney, liver, spleen, lung, intestine and rumen(P <0.01). Immunohistochemical results showed that AQP7 protein was mainly distributed in the muscle cells of the heart and muscles and the proximal convoluted tubules of the kidney, and its expression in the heart, muscles and kidney were higher than that in the liver, spleen, lung, intestine and rumen of Jiulong yak(P <0.05).This study may provide basic data for the physiological function and energy metabolism mechanism in yak high altitude adaptation.

Key words: Jiulong yak, Aquaporin 7, Tissue expression, Immunohistochemistry

CLC Number:

Q78
S823.03

LI Juan, ZHENG Yao, WANG Li. Identification of AQP7 Gene and the Expression and Location in Various Tissues of Jiulong Yak[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(4): 218-223. doi: 10.7668/hbnxb.20191828.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.7668/hbnxb.20191828

http://www.hbnxb.net/EN/Y2021/V36/I4/218

References

[1] Prieto-Martínez N, Vilagran I, Morató R, Rivera del Álamo M M, Rodríguez-Gil J E, Bonet S, Yeste M. Relationship of aquaporins 3(AQP3), 7(AQP7), and 11(AQP11) with boar sperm resilience to withstand freeze-thawing procedures[J]. Andrology, 2017, 5(6):1153-1164.doi:10.1111/andr.12410.
[2] Skowronski M T, Skowronska A, Rojek A, Oklinski M K, Nielsen S. Prolonged starvation causes up-regulation of AQP1 in adipose tissue capillaries of AQP7 knock-out mice[J]. International Journal of Molecular Sciences, 2016, 17(7):1101.doi:10.3390/ijms17071101.
[3] Iena F M, Lebeck J. Implications of aquaglyceroporin 7 in energy metabolism[J]. International Journal of Molecular Sciences, 2018, 19(1):154.doi:10.3390/ijms19010154.
[4] Fu X H, Zhu J, Zhang L, Shu J. Long non-coding RNA NEAT1 promotes steatosis via enhancement of estrogen receptor alpha-mediated AQP7 expression in HepG2 cells[J]. Artificial Cells, Nanomedicine, and Biotechnology, 2019, 47(1):1782-1787.doi:10.1080/21691401.2019.1604536.
[5] Liu Z J, Shen J, Carbrey J M, Mukhopadhyay R, Agre P, Rosen B P. Arsenite transport by mammalian aquaglyceroporins AQP7 and AQP9[J]. Proceedings of the National Academy of Sciences of the United States of America, 2002, 99(9):6053-6058.doi:10.1073/pnas.092131899.
[6] Rojek A, Praetorius J, Frøkiaer J, Nielsen S, Fenton R A. A current view of the mammalian aquaglyceroporins[J]. Annual Review of Physiology, 2008, 70:301-327.doi:10.1146/annurev.physiol.70.113006.100452.
[7] Lebeck J, Østergård T, Rojek A, Füchtbauer E M, Lund S, Nielsen S, Praetorius J. Gender-specific effect of physical training on AQP7 protein expression in human adipose tissue[J]. Acta Diabetologica, 2012, 49(S1):S215-S226.doi:10.1007/s00592-012-0430-1.
[8] Kishida K, Kuriyama H, Funahashi T, Shimomura I, Kihara S, Ouchi N, Nishida M, Nishizawa H, Matsuda M, Takahashi M, Hotta K, Nakamura T, Yamashita S, Tochino Y, Matsuzawa Y. Aquaporin adipose, a putative glycerol channel in adipocytes[J]. Journal of Biological Chemistry, 2000, 275(27):20896-20902.doi:10.1074/jbc.M001119200.
[9] Zheng D, Peng M Y, Zhu X N, Wang J. Effects of qutanhuoxue decoction on AQP7 and AQP9 expression in nonalcoholic fatty liver model rats[J]. Evidence Based Complementary and Alternative Medicine, 2019, 2019:5709626.doi:10.1155/2019/5709626.
[10] Fu X H, Xing L L, Xu W H, Shu J. Treatment with estrogen protects against ovariectomy-induced hepatic steatosis by increasing AQP7 expression[J]. Molecular Medicine Reports, 2016, 14(1):425-431.doi:10.3892/mmr.2016.5236.
[11] Gomes D, Agasse A, Thiébaud P, Delrot S, Gerós H, Chaumont F. Aquaporins are multifunctional water and solute transporters highly divergent in living organisms[J]. Biochimicaet Biophysica Acta, 2009, 1788(6):1213-1228.doi:10.1016/j.bbamem.2009.03.009.
[12] 毛进彬, 毛旭东, 代舜尧, 涂永强, 邓启红, 沈常欢.亚丁牦牛生长发育性能研究[J]. 中国草食动物科学, 2020, 40(5):74-76.doi:10.3969/j.issn.2095-3887.2020.05.017.
Mao J B, Mao X D, Dai S Y, Tu Y Q, Deng Q H, Shen C H. Research on the growth and development performance of Yading yak[J]. China Herbivore Science, 2020, 40(5):74-76.
[13] 贾功雪, 丁路明, 徐尚荣, 方有贵, 付弘赟, 杨其恩. 青藏高原牦牛遗传资源保护和利用:问题与展望[J]. 生态学报, 2020, 40(18):6314-6323.doi:10.5846/stxb201912232763.
Jia G X, Ding L M, Xu S R, Fang Y G, Fu H Y, Yang Q E. Conservation and utilization of yak genetic resources in Qinghai-Tibet plateau:Problems and perspectives[J]. Acta Ecologica Sinica, 2020, 40(18):6314-6323.
[14] Lebeck J, Søndergaard E, Nielsen S. Increased AQP7 abundance in skeletal muscle from obese men with type 2 diabetes[J]. American Journal of Physiology Endocrinology and Metabolism, 2018, 315(3):367-373.doi:10.1152/ajpendo.00468.2017.
[15] 张亚楠, 王永, 朱江江, 许晴, 林亚秋.藏山羊AQP7 基因克隆和不同组织器官差异表达分析[J]. 西南农业学报, 2019, 32(8):1956-1960.doi:10.16213/j. cnki. scjas. 2019. 8.042.
Zhang Y N, Wang Y, Zhu J J, Xu Q, Lin Y Q. Analysis of cloning and expression of AQP7 gene in Tibetan goat[J]. Southwest China Journal of Agricultural Sciences, 2019, 32(8):1956-1960.
[16] 郭榕. AQP7介导的人参皂苷Rb1改善肥胖的作用及其机制研究[D].福州:福建医科大学, 2018.
Guo R. AQP7 involved in ginsenoside-Rb1-mediated anti-obestiy through the activation of PPAR-γ pathway[D].Fuzhou:Fujian Medical University, 2018.
[17] Hara-Chikuma M, Satooka H, Watanabe S, Honda T, Miyachi Y, Watanabe T, VerkmanA S. Aquaporin-3-mediated hydrogen peroxide transport is required for NF-κB signalling in keratinocytes and development of psoriasis[J]. Nature Communications, 2015, 6:7454.doi:10.1038/ncomms8454.
[18] Chen Y H, Jin S, Teng X, Hu Z J, Zhang Z H, Qiu X, Tian D Y, Wu Y M. Hydrogen sulfide attenuates LPS-induced acute kidney injury by inhibiting inflammation and oxidative stress[J]. Oxidative Medicine and Cellular Longevity, 2018, 2018:6717212.doi:10.1155/2018/6717212.
[19] Vieceli Dalla Sega F, Zambonin L, Fiorentini D, Rizzo B, Caliceti C, Landi L, Hrelia S, Prata C. Specific aquaporins facilitate Nox-produced hydrogen peroxide transport through plasma membrane in leukaemia cells[J]. Biochimicaet Biophysica Acta, 2014, 1843(4):806-814.doi:10.1016/j.bbamcr.2014.01.011.
[20] 宋国强. 牦牛AQP4 基因的克隆及其对高原脑水肿抗性的表达机理[D].兰州:兰州大学, 2011.
Song G Q. AQP4 gene cloning and its expression mechanism of resistance to high altitude cerebral edema in the yak(Bos grunniens) [D].Lanzhou:Lanzhou University, 2011.
[21] 汪迁昊. 牦牛脑AQP9的功能特征[D].兰州:兰州大学, 2017.
Wang Q H. Functional characteristics of AQP9 in the yak brain[D].Lanzhou:Lanzhou University, 2017.
[22] Mourelatou R, Kostopoulou E, Rojas-Gil A P, Kehagias I, Linos D, Kalfarentzos F E, Spiliotis B E. Decreased adipocyte glucose transporter 4(GLUT4) and aquaglyceroporin-7(AQP7) in adults with morbid obesity:Possible early markers of metabolic dysfunction[J]. Hormones, 2019, 18(3):297-306.doi:10.1007/s42000-019-00130-8.
[23] Skowronski M T, Lebeck J, Rojek A, Praetorius J, Füchtbauer E M, Frøkiaer J, Nielsen S. AQP7 is localized in capillaries of adipose tissue, cardiac and striated muscle:Implications in glycerol metabolism[J]. American Journal of Physiology Renal-Physiology, 2007, 292(3):956-965.doi:10.1152/ajprenal.00314.2006.
[24] Li Y J, Wang W L, Jiang T, Yang B X. Aquaporins in urinary system[J]. Advances in Experimental Medicine and Biology, 2017, 969:131-148.doi:10.1007/978-94-024-1057-0_9.
[25] Guo M, Chen F, Lin T F, Peng Y Q, Li W P, Zhu X X, Lin L, Chen Y S. Apelin-13 decreases lipid storage in hypertrophic adipocytes in vitro through the upregulation of AQP7 expression by the PI3K signaling pathway[J]. Medical Science Monitor, 2014, 20:1345-1352.doi:10.12659/MSM.890124.
[26] Lebeck J. Metabolic impact of the glycerol channels AQP7 and AQP9 in adipose tissue and liver[J]. Journal of Molecular Endocrinology, 2014, 52(2):165-178.doi:10.1530/JME-13-0268.
[27] Karaca A, Palabıyık O, Taȿtekin E, Turan F N, Vardar S A. High fructose diet suppresses exercise-induced increase in AQP7 expression in the in vivo rat heart[J]. Anatolian Journal of Cardiology, 2016, 16(12):916-922.doi:10.14744/anatoljcardiol.2016.6958.
[28] Tie L, Wang D, Shi Y D, Li X J. Aquaporins in cardiovascular system[J]. Advances in Experimental Medicine and Biology, 2017, 969:105-113.doi:10.1007/978-94-024-1057-0_6.
[29] Oikonomou E, Kostopoulou E, Rojas-Gil A P, Georgiou G, Spiliotis B E. The metabolic implications of aquaporin 7(AQP7) promoter variants in lean children and children with obesity[J]. Hormones, 2020, 19(2):187-195.doi:10.1007/s42000-020-00184-z.
[30] He J Z, Yang B X. Aquaporins in renal diseases[J]. International Journal of Molecular Sciences, 2019, 20(2):366.doi:10.3390/ijms20020366.
[31] 贺大芳.牦牛和黄牛心脏、肺脏组织microRNA转录组的鉴定与差异表达分析[D].雅安:四川农业大学, 2017.
He D F.Identification and differential expression analysis of microRNAs in heart and lung tissues between yak and cattle[D].Ya'an:Sichuan Agricultural University, 2017.

Please choose a citation manager

Content to export