Cloning and Tissue Expression of Troponin I Gene Family in Yak

doi:10.7668/hbnxb.20190667

Abstract

Abstract: In order to clone the TNNI gene family, predict its protein structure and function, and analyze its expression differences in different yak tissues, this study used 0.5-year-old healthy Leiwuqi yak as the experimental material, and the CDS of the yak troponin I gene family was cloned by RT-PCR and then the sequences were analyzed using bioinformatics, the mRNA expression level of TNNI family genes were determined using Real-time PCR. The results showed that the CDS of TNNI1, TNNI2 and TNNI3 were 564, 549 and 639 bp, encoding 187, 182 and 212 amino acid, respectively. The results of predictive analysis showed that the proteins encoded by the TNNI gene family were all alkaline proteins, the protein structure was unstable, no transmembrane domain, no signal peptide, and non-secretory protein; the second and third-order structures were α-helix mainly, both contained the Troponin superfamily conserved domain. Phylogenetic tree analysis showed that the Leiwuqi yak TNNI1 gene had the closest associated with that of Bubalus bubalis, followed by Ovis aries and Bos taurus, and the furthest relationship with Mus musculus; TNNI2 and TNNI3 were closely related to Bos taurus and Bubalus bubalis, and distant with the others. The Real-time quantitative PCR results showed that the relative expression of TNNI1 and TNNI2 genes were the highest in the gluteal muscles, and the expression of TNNI1 in the heart, liver and lung was significantly higher than that of TNNI2 (P<0.05), the TNNI3 gene was expressed in the heart at a higher level, but the expression level was low in the lung, gluteal muscle and liver.

Key words: Yak, Troponin I gene family, Cloning, Tissue expression

CLC Number:

S823.8+5

XIANG Ya, CHAI Zhixin, WANG Jikun, XIN Jinwei, WANG Hui, WANG Jiabo, WU Zhijuan, ZHONG Jincheng, JI Qiumei. Cloning and Tissue Expression of Troponin I Gene Family in Yak[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(2): 228-238. doi: 10.7668/hbnxb.20190667.

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