Optimization of Correction Factor of Soil Nitrate Nitrogen by Ultraviolet Spectrophotometry

doi:10.7668/hbnxb.20190355

Abstract

Abstract: Soil nitrate nitrogen plays an important role in nitrogen nutrient management in dryland crops. Ultraviolet spectrophotometry for the determination of soil nitrate nitrogen has the advantages of simple operation, fast measurement and less interference factors, but it is favored by most laboratories. The correction factor in the spectrophotometry requires optimization due to the uncertainty of the extractant, wavelength selection, and soil type. Soil nitrate nitrogen was determined from eight soil types in different ecological regions from east to west in Inner Mongolia. The effects of extractant species, wavelength selection and soil type on the correction factor in UV spectrophotometry were studied. The results showed that the extractant and the wavelength had an important influence on the correction factor. There was no correlation between the correction factor values obtained by CaCl₂ and KCl extractant, and there was a significant correlation between the correction factor values at 210,220 nm. And the correction factor value at 210 nm was generally higher than 220 nm. Different soil types had different effects on correction factors for different extractants and wavelengths. The correction factors for KCl extracts at 210,220 nm were 3.4 and 2.8, respectively, while the CaCl₂ extracts had correction values of 4.4 and 2.9 at 210,220 nm, respectively. Compared with the phenol disulfonic acid method under different extracting agents and different wavelengths, the lowest RMSE and RE% were obtained by using KCl as the extractant at 220 nm wavelength (correction factor 2.8), Which was 1.8 and 15.6% respectively. The comparison between flow analysis and reflectometry further demonstrated that a KCl extractant with a correction correction factor of 2.8 at 220 nm was feasible.

Key words: Soil nitrate nitrogen, Phenol disulfonic acid method, Ultraviolet spectrophotometry, Correction factor

CLC Number:

S151.9+5

MIAO Jie, LI Fei, ZHANG Jiakang, WEI Bangtai, JIA Yuze, YANG Haibo. Optimization of Correction Factor of Soil Nitrate Nitrogen by Ultraviolet Spectrophotometry[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(S1): 204-212. doi: 10.7668/hbnxb.20190355.

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