Molecular Cloning, Temporal and Spatial Expression Patterns, and Subcellular Localization of Porcine TAK1 Gene

doi:10.7668/hbnxb.2018.05.009

Abstract

Abstract: To investigate the biological role of transforming growth factor-β-activated kinase 1 (TAK1) in porcine skeletal muscle development, we cloned the porcine TAK1 cDNA sequences using rapid amplification of cDNA ends (RACE) method. We also detected the temporal and spatial expression patterns and subcellular localization of porcine TAK1 gene. The full-length cDNA sequence of TAK1 was 2 163 bp (GenBank No. KU504629), including a 1 740 bp open reading frame (ORF) that encoded a putative 579 amino acid, and the high sequence similarity of TAK1 between the pig and human, or monkey and or sheep were 98.8%. TAK1 contained a conserved serine/threonine protein kinase catalytic domain (STKc_TAK1), an activation loop (A-Loop), some ATP banding sites and TAB1(TGF-beta activated kinase 1)banding sites. The highly conserved domains indicated that TAK1 had a similar function. The tissue analysis showed that the TAK1 mRNA expression was the highest in pig spleen tissue and very low in pancreas and longissimus dorsi muscle tissue. The result of different developmental stages expression analysis indicated that TAK1 mRNA had the highest expression in embryonic and down-regulation during longissimus dorsi muscle development. Comparing the different breeds, the mRNA abundance of TAK1 in Large White was higher than in Meishan pigs at all development stages, whereas at 90 days, there was no significant difference (P >0.05). Moreover, the fluorescence co-localization showed that the expression of TAK1 was observed mainly in the cytoplasm. These results suggested that TAK1 was probably playing a key role in porcine skeletal muscle development, and made a foundation for the further study on the function of TAK1 in pigs.

Key words: Pig, TAK1, Skeletal muscle, Clone, Expression, Subcellular localization

CLC Number:

S828

LI Rui, XU Haixia, WU Wei, WANG Suying, REN Mengke, ZHANG Pengpeng, XU Yongjie. Molecular Cloning, Temporal and Spatial Expression Patterns, and Subcellular Localization of Porcine TAK1 Gene[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2018, 33(5): 68-75. doi: 10.7668/hbnxb.2018.05.009.

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