ACTA AGRICULTURAE BOREALI-SINICA ›› 2016, Vol. 31 ›› Issue (1): 15-21. doi: 10.7668/hbnxb.2016.01.003

Special Issue: Cotton Biotechnology

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Genome-wide Analysis of the EPSPS Genes in Upland Cotton

GONG Yuanyong, XU Zhenzhen, GUO Shuqiao, SHU Hongmei, JIANG Lu, NI Wanchao   

  1. Jiangsu Academy of Agricultural Sciences, Key Laboratory of Cotton and Rapeseed, Ministry of Agriculture, Nanjing 210014, China
  • Received:2015-11-09 Published:2016-02-28

Abstract: Currently,two EPSPS genes from Gossypium hirsutum have been submitted to NCBI.It is more convinent to identify EPSPS genes in genome level of upland cotton comprehensively following the publication of Gossypium hirsutum genomic sequence.Four EPSPS homologous genes were identified from the genome sequence database of allotetraploid cotton (Gossypium hirsutum L.acc.TM-1),and they were found to be distributed on subgenomes of A07,A12,D07 and D12.Under the comperation of nucleotide sequences,amino acid sequences and gene structures,phylogenetic analysis of constructing phylogenetic tree and the study of transcription situation of these four genes,it was more clearly that the two genes located on A07 and D07 subgenomes belong to highly homologous co-linear gene,and the two genes located on A12 and D12 subgenomes as well.Sequence alignment results showed that the two EPSPS genes submitted to NCBI were the two genes located on A12 and D12 subgenomes respectively.The study results of this paper would provide an theoretical basis on cloning and functional research of EPSPS genes which located on A07 and D07 subgenomes.

Key words: Gossypium hirsutum, Genome sequence of upland cotton, EPSPS gene

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Cite this article

GONG Yuanyong, XU Zhenzhen, GUO Shuqiao, SHU Hongmei, JIANG Lu, NI Wanchao. Genome-wide Analysis of the EPSPS Genes in Upland Cotton[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2016, 31(1): 15-21. doi: 10.7668/hbnxb.2016.01.003.

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