Abstract: In order to investigate the effect of orexins A on the energy metabolism,growth and development of sheep ovary,the luteinising granulosa cells were isolated and cultured in vitro.The passage luteinising granulosa cells were treated with 0.05,0.10 μg/mL of orexins A,and the total proteins of the cells were extracted at 0,2,6,12,24 h treatment,respectively.The relative expression of P-AMPK and P-ERK were determined by Western Blot.The results showed that the expression of P-AMPK of the cells treated with 0.05,0.10 μg/mL of orexins A 2 h after treatment were significantly upregulated compared with that of control (P<0.01),respectively.The expression of P-AMPK of the cells treated with 0.05,0.10 μg/mL of orexins A 6 h treatment reached the extreme value (P<0.01),respectively,and expression of P-AMPK of the cells treated with 0.10 μg/mL of orexins A were significantly higher than that of the 0.05 μg/mL treated group.The expression of P-ERK of the cells treated with 0.05,0.10 μg/mL of orexins A 2 h treatment were significantly upregulated compared with that of control (P<0.01),respectively.The expression of P-ERK of the cells treated with 0.10 μg/mL of orexins A 24 h treatment were significantly higher than that of the plateau phase (6-12 h) and reached the extreme value(P<0.01).It was indicated that the orexins A was involved in the regulation of reproductive function by regulating the energy metabolism,growth and development of sheep throughout the signaling pathway of P-AMPK and P-ERK.

Key words: Orexin A, Granulosa cells, P-AMPK, P-ERK

CLC Number: 

• S826