Construction and Effect Test of the Eukaryotic Co-expression Vector for Myogenin and Myf5

doi:10.7668/hbnxb.2014.02.003

Abstract

Abstract: Muscle development was specially controlled by the myogenic factors such as myogenin and Myf5. It is necessary to cotransfect these two myogenic factors into non-muscle cells for further investigating the machanism by which they regulate the muscle specific genes. To gain high transfection efficiency, the coding sequences of myogenin and Myf5 were cloned successively into the pVITRO2, an expression vector containing two multiple cloning sites (MCS) for the convenient cloning of two cDNAs. Sequencing results proved that the cloned myogenin and Myf5 are correct, and the transfection assay suggested they could activate their respective target genes respectively.

Key words: Myogenesis, Co-expression vector, Myogenic factors

CLC Number:

DU Chao, SUN Hong-min, WANG Ying-ze, SUN Guo-jie. Construction and Effect Test of the Eukaryotic Co-expression Vector for Myogenin and Myf5[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2014, 29(2): 13-17. doi: 10.7668/hbnxb.2014.02.003.

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