ACTA AGRICULTURAE BOREALI-SINICA ›› 2013, Vol. 28 ›› Issue (5): 44-47. doi: 10.7668/hbnxb.2013.05.008

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Expression of a Serine Carboxypeptidase HoSCP of Holotrichia oblita( Coleoptera: Scarabaeoidea) in Different Systems

ZHANG Ya-kun1, ZHAO Dan3, GUO Wei1,2,3, LIU Xiao-min4, XU Da-qing1, SUN Wei-ming3   

  1. 1 College of Life Sciences, Agricultural University of Hebei, Baoding 071001, China;
    2 Plant Science and Technology College, Beijing University of Agriculture, Beijing 102206, China;
    3 College of Plant Protection, Agricultural University of Hebei, Biological Control Center of Plant Diseases and Plant Pests of Hebei Province, National Engineering Research Center for Agriculture in Northern Mountainous Areas, Baoding 071001, China;
    4 Institute of Cereal and Oil Crops, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050035, China
  • Received:2013-07-17 Published:2013-10-28

Abstract: Serine carboxypeptidases ( SCPs ) are a class of protease enzyme that play essential roles in a wide range of biological processes which are the modification and degradation of peptides and proteins. We report,in this study,the hoscp gene of Holotrichia oblita was amplified by PCR method and cloned into the expression vector pET 28b and pPIC9K,respectively. The following recombinant vector was transformed into Escherichia coli BL21 and Pichia pastoris GS115,respectively. The results showed that HoSCP was stably expressed as a 50 kDa recombinant protein in E.coli and P.pastoris.It is useful for the further study of activity and biological function of HoSCP in H.oblita.

Key words: Holotrichia oblita, SCPs, HoSCP, Prokaryotic expression system, P. pastoris expression system

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Cite this article

ZHANG Ya-kun, ZHAO Dan, GUO Wei, LIU Xiao-min, XU Da-qing, SUN Wei-ming. Expression of a Serine Carboxypeptidase HoSCP of Holotrichia oblita( Coleoptera: Scarabaeoidea) in Different Systems[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2013, 28(5): 44-47. doi: 10.7668/hbnxb.2013.05.008.

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