Influence of Epidermal Growth Factor on Cell Proliferation Cycle and Apoptosis of Cultured Bovine Oviductal Epithelial Cells in vitro

doi:10.3969/j.issn.1000-7091.2012.z1.080

Abstract

Abstract: To investigate the effect of epidermal growth factor ( EGF) on proliferation， cell cycle and apoptosisof cultured bovine oviductal epithelial cells and provide foundation for further study of system of co-culturing bovineembryo in vitro． The cultured bovine oviductal epithelial cells were treated with different dose of EGF． The effects ofbovine oviductal epithelial cells proliferation， cell cycle and apoptosis were measured by blood counting assay andanalyzed by FCM ( flow cytometry) and Lysis software． In the definite range of EGF (0 － 50 ng /mL) ， the effect oncells increased with raise of the EGF concentration showed dose-dependently． With the concentration improved， theabilities of promoting proliferation and inhibiting apoptosis had decreased． Besides， cell quantities increased obviously at S phase while declined slightly at G₂-M phase． In the definite range of concentration， the epidermal growth factor could promote the proliferation and inhibiting apoptosis of the cultured bovine oviductal epithelial cells， moreover， the cell cycles had been altered after adding EGF．

Key words: Bovine oviductal epithelial cell, EGF, Cell proliferation, Cell cycle, Cell apoptosis

CLC Number:

S823

SUN Wei, LI Xi-he, GUO Ji-tong, BA Te-er, YANG Li-guo. Influence of Epidermal Growth Factor on Cell Proliferation Cycle and Apoptosis of Cultured Bovine Oviductal Epithelial Cells in vitro[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(S1): 410-414. doi: 10.3969/j.issn.1000-7091.2012.z1.080.

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