Molecular Cloning and Sequence Analysis of CbPG Gene from Capsella bursapastoris (L.) Medic.

doi:10.3969/j.issn.1000-7091.2012.05.003

Abstract

Abstract: In the paper, CbPG gene, homologous with the BcMF2 gene ( EUI81170 ) encoding pollen-specific polygalacturoase (PG) of Chinese cabbage,was cloned from Capsella bursapastoris ( L. ) Medic. by PCR amplication technique. The CbPG gene showed 99% overall DNA similarity with that of BcMF2. The gene consists of 1 563 bp encoding a protein of 420 amino acids, interrupted by three introns. Sequence alignment revealed that four domains which are highly conserved in the all plant and fungal PGs is present in CbPG. Phylogenetic analysis showed that CbPG fails into the category of clade-C,which includes PGs related to pollen development. These results test that the CbPG gene might also acts as pollen-specific polygalacturonase.

Key words: CbPG, Cloning, Sequence features, Bioinformatic analysis

CLC Number:

S636.9

ZHANG Tao. Molecular Cloning and Sequence Analysis of CbPG Gene from Capsella bursapastoris (L.) Medic.[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(5): 12-17. doi: 10.3969/j.issn.1000-7091.2012.05.003.

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