Cloning and Expression Characterization of Ethylene Receptor Gene Cm-ETR2 from Melon (Cucumis melo L．)

doi:10.3969/j.issn.1000-7091.2012.02.008

Abstract

Abstract: A pair of specific primer was designed based on melon ethylene receptor gene Cm-ETR2 in Gen- bank． The full-length cDNA of Cm-ETR2 was cloned by RT-PCR from ripening fruit of melon(Cucumis melo L． cv． Hetao)． The length of cDNA was 2 304 bp，and the cDNA encodes 767 amino acids． Sequence analysis indicated that the cDNA sequence shared high similarity to that of the melon ETR2 reported previously． And the deduced amino acid sequences are consistent with those of the melon reported previously． The expression characterization of these genes during melon fruit development and ripening was analyzed by real-time quantitative RT-PCR． The results indi- cated that there were no changes of the expression level of ETR2 from 15 DAP to 30 DAP． The expression level began to increase at 35 DAP and the increasing continued until 45 DAP． The highest level was 9 times than 15 DAP．

Key words: Melon, Ethylene receptor, ETR2, Fruit ripening, Expression

CLC Number:

S652.1

HAO Jin-feng, WANG Xue-fei, YI Rong, HASI Agula, NIU Yi-ding. Cloning and Expression Characterization of Ethylene Receptor Gene Cm-ETR2 from Melon (Cucumis melo L．)[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(2): 40-43. doi: 10.3969/j.issn.1000-7091.2012.02.008.

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