Abstract: GDF11, also known as bone morphogenetic protein 11(BMP11), is a member of the transforming growth factor-β superfamily and BMPs subfamily. It is a secreted protein which is considered to be a key regulator of axial skeleton patterning during early embryogenesis. In this study, the partial cDNA of porcine GDF11 gene was cloned by RT-PCR and the expression patterns of GDF11 gene were detected. Sequence analysis indicates that the sequences of porcine GDF11 gene were 1161 bp in length(GenBank: HQ657211. 2). The cloned cDNA encoded a protein of 332 amino acid residues, which shared 99. 10%, 99. 10%, 99. 10%, 99. 40%, 99. 10%, 98. 80% and 78. 82% identity with those of human, mouse, rat, cattle, horse, rabbit and zebra fish, respectively. Expression patterns of GDF11 were detected in 14 different tissues by real time quantitative RT-PCR, and patterns of GDF11 gene, as a whole, tended to be spinal cord>backfat>urinary bladder>lung>spleen>gallbladder>kidney>large intestine>heart>skeletal muscle>liver>small intestine>longissimus Dorsi muscle>stomach. These results provide important information for further elucidating the molecular biology functions and mechanism of GDF11 gene.

Key words: Licha black pig, GDF11 gene, Gene cloning, Real time quantitative RT-PCR, Tissue expression patterns

CLC Number: 

• S828

• Q789