Over-expression Vector Construction and Transgenic Soybean(Glycine maxL) Plants Identification of Transcription Factor Gene GmPTF1

doi:10.7668/hbnxb.2011.06.006

Abstract

Abstract: The over-expression vector pGN-GmPTF1and marker-free expression vector pX6-GmPTF1were constructed in this study base on the cloning and identification of GmPTF1，a soybean high phosphorus-efficiency transcription factor gene，and the vectors were introduced into different soybean varieties(Ji-dou 12， Ji-dou 16，Wu-xing 1) by Agrobacterium-mediated cotyledonary-node and pollen tube pathway transformation methods． PCR analysis results of transformation plants showed that the GmPTF1was successfully incorporated into the soybean genome． Total five PCR positive plants of vector pGN-GmPTF1and six PCR positive plants of vector pX6-GmPTF1were obtained from T₀generation， respectively．

Key words: Soybean, Transcription factor, Vector construction, Genetic transformation

CLC Number:

ZHENG Xing, ZHANG Cai-ying, CHANG Wen-suo, LI Xi-huan. Over-expression Vector Construction and Transgenic Soybean(Glycine maxL) Plants Identification of Transcription Factor Gene GmPTF1[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2011, 26(6): 27-31. doi: 10.7668/hbnxb.2011.06.006.

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http://www.hbnxb.net/EN/Y2011/V26/I6/27

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