Genetic Variations in CACNA2D1 Gene and Its Association with Slaughter Traits in Chinese Simmental

doi:10.7668/hbnxb.2011.04.009

ACTA AGRICULTURAE BOREALI-SINICA ›› 2011, Vol. 26 ›› Issue (4): 50-60. doi: 10.7668/hbnxb.2011.04.009

Special Issue: Animal husbandry； Biotechnology；

Genetic Variations in CACNA2D1 Gene and Its Association with Slaughter Traits in Chinese Simmental

ZHANG Meng¹, ZHANG Li-min¹, ZHOU Zheng-kui^1,2, YUAN Zheng-rong¹, HUANG Meng^1,2, DENG Gui-xin¹, LIU Xi-dong³, LIU Li¹, LI Jiao¹, GAO Xue¹, GAO Hui-jiang¹, XU Shang-zhong¹, LI Jun-ya¹

1. Institute of Animal Science, Beef Cattle Research Center, Chinese Academy of Agricultural Sciences, Key Laboratory of Farm Animal Genetic Resources and Utilization of Ministry Agricultury, Beijing 100193, China;
2. College of Animal Science and Technology, Northwest Agricultural and Forestry University, Yangling 712100, China;
3. College of Animal Science and Technology, Northeast Agricultural University, Haerbin 150030, China

Received:2011-05-14 Published:2011-08-28

Abstract

Abstract: The objective of the study was to identify genetic polymorphisms of the CACNA2D1 gene and to analyze associations between SNPs and slaughter traits in Chinese Simmental.In the study,it adopted recently developed SNPS chip technology,differed from the traditional PCR-SSCP or PCR-RFLP technology,to detect the genetic variation of CACNA2D1 gene in a population with 136 individuals.The results showed that CACNA2D1 gene existed 12 effective SNPs totally,Genetic indices(Ho,Ne and PIC)were presented low genetic diversity on 4 loci,the other eight loci were highly polymorphic,and 10 were in HarDY-Weinberg equilibrium(P0.05).The results of the association analysis showed that locus 1 different genotypes of individual differed in meat color trait significantly(P0.05),locus 2 different genotypes of individual in thigh meat thick differed significantly(P 0.05),locus 3 different genotypes of individual differed in carcass weight,dressing percentage,marbling and thighs meat thick several important slaughter traits significantly(P 0.05),while in meat color trait reached extremely significant(P 0.01).It was concluded that locus 3 heterozygote was thought to be the dominant genotype,so was AA on other loci.Therefore,these results suggest that CACNA2D1 gene is a candidate gene that affects slaughter traits,especially locus 3 can serve as a useful marker for slaughter traits in Chinese Simmental.

Key words: Chinese Simmental, CACNA2D1 gene, SNPs chip, Slaughter traits, Association analysis

CLC Number:

S823

ZHANG Meng, ZHANG Li-min, ZHOU Zheng-kui, YUAN Zheng-rong, HUANG Meng, DENG Gui-xin, LIU Xi-dong, LIU Li, LI Jiao, GAO Xue, GAO Hui-jiang, XU Shang-zhong, LI Jun-ya. Genetic Variations in CACNA2D1 Gene and Its Association with Slaughter Traits in Chinese Simmental[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2011, 26(4): 50-60. doi: 10.7668/hbnxb.2011.04.009.

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