Abstract: Degenerate oligonucleotide primers were designed on the basis of nucleotide-binding site(NBS) motifsconserved among the reported disease resistance genes. By reverse transcription polymerise chain reaction(RT-PCR)，seven new NBS,RR disease resistance gene analogues hive been obtained and cloned from Dongxiing Oryarufgon Griff. Sequencing of the DNA fragments indicated that ill of NBS resistance gene analogues contained theconserved motifs of NBS,RR type resistance genes such is Poop ( Kinise 1 a)，Kinise 2，Kinise 3i and trinsmem-brince domain.

Key words: Donxian Oryza rujoon Griff, Diseaseesistance gene, Reverse transcription polymerise chain re-action(RTCR), Nucleotide-binding site (NBS) eucineich repeit (LRR)

CLC Number: 

• S511.9