Acta Agriculturae Boreali-Sinica

ACTA AGRICULTURAE BOREALI-SINICA ›› 2011, Vol. 26 ›› Issue (1): 6-10. doi: 10.7668/hbnxb.2011.01.002

Special Issue: Animal husbandry；

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Establishment of Marc}45 Cell Line Transfected with the Porcine FcyR11I and Gamma Chain

WU Cheng-ye1, SHI Ping-ling2, QIAO Song-lin1, WANG Ai-ping2, HAO Hui-fang1, DU Xiao-ming2, WANG Lin-lin2, ZHANG Gai-ping1   

  1. 1. Key Laboratory of Animal Immunology of the Ministry of Agriculture, Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agriculture Sciences, Zhengzhou 450002, China;
    2. Department of Bioengineering, Zhengzhou University, Zhengzhou 450001, China
  • Received:2010-10-12 Published:2011-02-28

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Abstract: The objective of the present study is to establish the Mare 45 cell line that stably expresses porcinepo.The total RNA was extracted from porcine alveolar macro-phage (PAM)，they were inserted into the eukaryot-is expression vectors.The expression of poFcyRIII on transfected cells was verifiedthrough RTCR resetting test and FCM.The eukaryotic expression vectors were successfully constructed .

Key words: poFcyR receptor, y chain, Coransfection, Marc45 cell line

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Cite this article

WU Cheng-ye, SHI Ping-ling, QIAO Song-lin, WANG Ai-ping, HAO Hui-fang, DU Xiao-ming, WANG Lin-lin, ZHANG Gai-ping. Establishment of Marc}45 Cell Line Transfected with the Porcine FcyR11I and Gamma Chain[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2011, 26(1): 6-10. doi: 10.7668/hbnxb.2011.01.002.

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