Isolation and Identification of Resistance-related Genes for Fusarium Wilt in Cucumber

doi:10.7668/hbnxb.2010.01.008

Abstract

Abstract: Fusarium wilt(Fusarium oxysporum f.sp.cucumerinum Owen.) is one of major diseases that cause significant disease losses in cucumber.It is significant to explore the disease resistance molecular mechanism against Fusarium wilt race 4 in cucumber breeding.The cucumber resistant line Cu14 were employed for studying on resistant genes expressing against Fusarium wilt by differential display reverse transcriptase PCR(DDRT-PCR) method.One fragment of cDNA expressed specially was got from the root-stem inoculated in cucumber,and was named as A178-2 with the size of 328 bp.Blast analysis showed that 145 bp nucleotide sequences of A178-2 was homologous with the generic grain protein in Arabidopsis and reached 85%.Further studying on expression of the gene will be done.

Key words: Cucumber, Fusarium wilt race 4, Disease resistance-related genes, Isolation, Identification

CLC Number:

SHEN Feng-rui1,2,WU Ping1,MAO Ai-jun1,ZHOU Hong-mei1,WANG Yong-jian1,YU Shuan-cang1,ZHANG Li-rong1,YANG Yan-li2,ZHENG Xiao-ying1. Isolation and Identification of Resistance-related Genes for Fusarium Wilt in Cucumber[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(1): 40-43. doi: 10.7668/hbnxb.2010.01.008.

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