Cloning and Analysis of a Chitinase Gene from Beauveia bassiana HFW-05

doi:10.7668/hbnxb.2010.01.007

Abstract

Abstract: To study characters of chitinase from Beauveria bassiana HFW-05 highly toxic to Bemisia tabaci and Plutella xylostella,the chitinase gene HFWBbchit1 was firstly cloned from Beauveria bassiana HFW-05.According to the published sequences of chit1 genes(EU828354),a pair of primers was designed for full length DNA cloning of HFWBbchit1 gene by PCR.Subsequently,the amplified fragment of HFWBbchit1 gene was inserted into Escherichia coli cloning vector pMD19-T and sequenced.Sequence analysis revealed a DNA sequence with a length of 1 047 bp and encoded an open reading frame consisting of 348 amino acids of 70 kDa peptide.The analysis shows that the gene sequence exhibited 98% identity to that of Beauveria bassiana NCIM1216 chitinase(EU828354)and Beauveria bassiana Bb0062(AY145440).The deduced amino acid sequence exhibited 99% identity to that of Beauveria bassiana Bb0062 chitinase(AAN41259)respectively.

Key words: Beauveria bassiana, Chitinase gene, Cloning

CLC Number:

S182
S76+.12

ZHEN Wei1,2,DU Li-xin2,CAO Wei-ping2,WANG Rong-yan2,SONG Jian2,WANG Jin-yao2,FENG Shu-liang2. Cloning and Analysis of a Chitinase Gene from Beauveia bassiana HFW-05[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(1): 36-39. doi: 10.7668/hbnxb.2010.01.007.

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http://www.hbnxb.net/EN/Y2010/V25/I1/36

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