Studies on Wheat Genetic Transformation Mediated Via Agrobacterium -tumefaciens in Which the Mature Embryos to be Explants

doi:10.7668/hbnxb.2009.S1.014

Abstract

Abstract: In this study,the genetic transformation procedure of wheat mediated via Agrobacterium -tumefaciens in which the mature embryos to be the explants was established.Under selection pressure condition,the generation rate of resistant calli to the transformed total mature embryos was 57.50 %,with a differentiation rate of resistant buds to the transformed total mature embryos being 8.58 %, and a plant generation rate of generated plants to the transformed total mature embryos being 0.83 %.In the tested three generated plants,PCR amplifications for the reporter gene Gus were all positive.In the meantime,the Gus activities in the plants were all significantly higher than the control (non -transformed plants).These results demonstrated that the tested plants generated from the transformed mature embryos were transgenic. Therefore,the genetic transformation approach established in this study would provide a powerful method for establishment of transgenic wheat lines in the future.

Key words: Wheat(TriticumaestivumL.), Matureembryos, Genetictransformation, Molecularidentification

CLC Number:

S512.03

GUOLi, GUJun-tao, LONGSu-xia, GUOCheng-jin, XIAOKai. Studies on Wheat Genetic Transformation Mediated Via Agrobacterium -tumefaciens in Which the Mature Embryos to be Explants[J]. ACTA AGRICULTURAE BOREALI-SINICA, doi: 10.7668/hbnxb.2009.S1.014.

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