Expression of MPB64 Protein from Mycobacterium bovis in Eukaryotic System

doi:10.7668/hbnxb.2009.05.045

Abstract

Abstract: The mpb64 gene fragment amplified by PCR from Mycobacterium bovis was cloned into the eukaryotic expression vector pcDNA3.1(+),and the recombinant plasmid pCM64 was obtained.Then the recombinant plasmid was transfected into SP2/0 cells in vitro.The expression of target gene was detected by indirect immunofluorescence and Western blotting.The result showed that the green fluorescence appeared in the SP2/0 cells transfected by pCM64 and the specific band about 23 kDa appeared in the electophoretogram of SP2/0 cells,indicaing that the target gene mpb64 had been successfully expressed.

Key words: Mycobacterium bovis, mpb64 gene, Expression

CLC Number:

S832

GONG Qiang, QU Ning, LIU Si-guo. Expression of MPB64 Protein from Mycobacterium bovis in Eukaryotic System[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2009, 24(5): 214-216. doi: 10.7668/hbnxb.2009.05.045.

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http://www.hbnxb.net/EN/Y2009/V24/I5/214

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