Construction and Analysis of AFLP Molecular Linkage Map in Pear

doi:10.7668/hbnxb.2009.03.038

Abstract

Abstract: Genetic linkage map was the foundation of locating quantitative trait loci,genes map-based cloning and marker assisted selection, while the research had done little on the pear. In order to accelerate the molecular breeding study on pear,a genetic map for pear( Pyrus L. ) has been developed based on 92 F1plants derived from an interspecific cross between the cultivars Zaomeisu and Bayuehong. The linkage map was constructed based on 208 polymorphic AFLP markers and the ″ double pseudo-testcross″strategy by using Jionmap version 3. 0 software. The map in this study covered 618. 7 cM of pear genome harboring 145 AFLP markers on the twenty linkage groups,which the length of linkage group varied from 2. 06 cM to 79. 3 cM and an average genetic distance between adjacent markers was about 6. 58 cM of Kosambi genetic distance. And also the markers on the linkage group varied from 4 to 20. Compared with the reports of pear genetic maps,the pear linkage map in this study was not saturated, but it was currently the first integrated linkage map in pear comprising 47 3∶ 1 and 98 1∶ 1AFLP markers by using Joinmap 3. 0 setting CP( cross pollination) type data.

Key words: Pyrus L., Molecular linkage map, Amplified fragment length polymorphic

CLC Number:

S661.203

SUN Wen ying, ZHANG Yu xing, LI Xiu gen, WANG Long. Construction and Analysis of AFLP Molecular Linkage Map in Pear[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2009, 24(3): 179-183. doi: 10.7668/hbnxb.2009.03.038.

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References

[1] Zabeau M,Vos P.Selective restriction fragment amplification:A general method for DNA fingerprinting[M].European Patent Application 92402629.7(Publication No.0534858A1).Paris:European Office,1993.
[2] 王斐,林盛华,方成泉,等.梨新品种及其亲本的AFLP分析[J].园艺学报,2007,34(4):847-852.
[3] 王艳芳,朱立武,贾兵,等.砀山酥梨AFLP技术体系建立及变异检测[J].安徽农业大学学报,2006,33(3):360一363.
[4] 马明臻.梨品种AFLP指纹图谱的构建[D].保定:河北农业大学,2005.
[5] Iketani H,Abe K,Yamnmoto T,et al.Mapping of the disease-related genes in Japanese pear using a molecular linkage map with RAPD markers[J].Breeding Science,2001,51:179-184.
[6] Yamamoto T,KiInura T,Shoda M,et al.Genetic linkage maps constructed by using an interspecific cross between Japanese and European pears[J].Theor Ape Genet,2002,106:9-18.
[7] Dondini L,Pierantoni L,Gaiotti F,et al.Identifying QTLs for fire-blight resistance via a European pear(Pyrus communis L.)genetic linkage map[J].Molecular-Breeding,2004,14(4):407-418.
[8] 鲁风娟.梨(Pyrus L.)AFLP反应体系的建立及在品种鉴定中的应用[D].保定:河北农业大学,2004.
[9] Hemmer M,Weeden N F,Mangands A G.Molecular marker linkage map for apple[J].J Heredity,1994,85:4-11.
[10] 张开春,李荣旗,毕晓颖,等.RAPD技术鉴定无融合生殖型平邑甜茶的有性后代[J].农业生物技术学报,1997,5(4):392-396.
[11] 杨朝东,张俊卫,熊彩凤,等.AFLP技术对梅花杂交种的快速鉴定[J].北京林业大学学报,2004,26(增刊):45-47.
[12] 鹿金颖,毛永民,申莲英,等.用AFLP分子标记鉴定冬枣自然授粉实生后代杂种的研究[J].园艺学报,2005,32(4):180-183.
[13] 王美,张凤兰,孟祥栋,等.中国白菜AFLP分子遗传图谱的构建[J].华北农学报,2004,19(1):28-33.
[14] 房经贵,刘大钧,马正强.利用双杂合位点标记资料构建芒果遗传图谱[J].分子植物育种,2003,1(3):313-319.
[15] Castiglioni P,Ajmone-Marsan P,Van Wijk R.AFLP markers in amolecularl Linkage map of maize:codominant scoring and lipkage group distribution[J].Theor Appl,Genet,1999,99:425-431.

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