Abstract:
Two pairs of specific primes were designed on the special sequences of Y chromosome and sharing DNAsequence of bull and a set of polymerize china reaction were used for identify the bull and cow blood samples andembryo. Results showed,only demonstrated a band of 250 by and bull demonstrated two band of 131 by by and 250bp, result`s of blood identification is completely in accordance with the actual sex type, 4 to 10 embryo cell also demon-strated same band model of blood sample, which shaved that this method of sex identification were effective andsensrtrve.
Key words:
Bovine embryo,
Sex identification,
Polymerize chain reaction
CLC Number:
ZHANG Li, SHI Ai-hua, LI Gui-ping, YU Wen-li. Sex Identification of Bovine Embryo with PCR Methods[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2009, 24(1): 212-214. doi: 10.7668/hbnxb.2009.01.047.