Inheritance and Expression of the Bt and npt II Genes in Transgenic Cotton

doi:10.7668/hbnxb.2008.06.037

Abstract

Abstract: Plasmid p G4AB containingBtgene was transferred into cotton Zhong 23 via pollon tube pathway.The Kanamycin detection and the expression ofBtICP,PCR were used to analyze the self-pollination generations from transgenic cotton Zhong 23.The results were as follows.ExogenousBtgene was amplified and expressed in the Kanamycin-resistance transgenic cotton.TheBtgene andnpt IIgene were inserted into the transgenic cotton genome,they were linked and inherited stably in different generations.npt IIgene can be used in inheritance analysis ofBtgene.

Key words: Btgene, npt IIgene, Inheritance, Expression

CLC Number:

S562

YAN Xi-zhong, ZHANG Rui, MENG Zhi-gang, SUN Guo-qing, ZHOU Tao, GUO San-dui. Inheritance and Expression of the Bt and npt II Genes in Transgenic Cotton[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2008, 23(6): 161-163. doi: 10.7668/hbnxb.2008.06.037.

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http://www.hbnxb.net/EN/Y2008/V23/I6/161

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