Enhancing Effects of Fluorescent Brightener 28 and Enhancin on Mythimna separata Nucleopolyhedrovirus

doi:10.7668/hbnxb.2008.04.006

Abstract

Abstract: The disruptive effects of Fluorescent Brightener 28( FB28) and Heliothis armigera granulovirus enhancing ( En -Ha) on the peritrophic membrane( PM) of larvae and enhancing effectsonMsNPV in Mythima separata were studied through SDS -PAGE, structural observation and bioassay.The results indicated that proteins could be dissociated from the PM in vitro by FB28.After the larvae were reared with diets containing 1% FB28 for 4 h, bluish fluorescence appeared on the disrupted PM.SDS -PAGE of PM treatedwith FB28 in vivo showed that some proteins with high molecular weight disappeared.After the PM was treated with En -Ha in vitro, some proteins with high molecular weight disappeared and some with lower molecular weight appeared, which indicated En -Ha could degrade the high -molecular -weight proteins from the PM.The PM became fragile after the larvaewere rearedwith diets containing 9μg/mL En -Ha for 7h, and SDS -PAGE showed that some proteinswith high molecular weight disappeared similar to the profile treated by FB28?However, the ex - periments testified that the disruptive effect could be restored after rearing the larvae on the diet without FB28 and En - Ha.The bioassay showed that FB28 and En -Ha could enhance the vitality of MsNPV and significantly decreased LC 50 by degrading the integrity of PM.

Key words: Fluorescent Brightener 28, Enhancin, MsNPV, Peritrophic membrane, Enhancing effect

CLC Number:

S432.4+1

CHEN Tao, WANG Jun-ping, LI Shu-wen, ZHOU Hong-xu, LI Guo-xun. Enhancing Effects of Fluorescent Brightener 28 and Enhancin on Mythimna separata Nucleopolyhedrovirus[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2008, 23(4): 23-27. doi: 10.7668/hbnxb.2008.04.006.

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