ACTA AGRICULTURAE BOREALI-SINICA ›› 2007, Vol. 22 ›› Issue (1): 156-160. doi: 10.3321/j.issn:1000-7091.2007.01.037

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Studies on Identification and Culture Characteristic of Alternaria obpyriformis T Y Zhang

LU Hong-xue1,2, ZHAO Ming-min1,2, LI Jian-qiang3   

  1. 1. Key Laboratory of Water-logged Disaster and Wetland Agriculture, Jingzhou 434025, China;
    2. College of Agriculture, Yangtze University, Jingzhou 434025, China;
    3. Hubei Provincial Seeds Group Company, Wuhan 430070, China
  • Received:2006-07-04 Published:2007-02-28

Abstract: In order to illuminate the occur rule of aloe black spot, the morphology identification and cultural characteristic of its pothogen Alternaria obpyriformis T Y Zhang by tissue isolating method, hemocytometer measurement method and suspension drop method. The results showed that the pathogen of aloe black spot was Alternaria obpyriformis T Y Zhang. The temperature ranging from 10 to 30℃ and the pH valure ranging from 3.92 to 10.82 was available for mycelia growth and conidial generation. The optimum temperature is 25℃. The optimum pH value for mycelia growth was 3.92-6.83. The optimum pH value for conidial generation was 5.23-6.83. The temperature ranging from 15 to 35℃ was suitable for conidial germination, the optimum temperature was 20-30℃. The optimum pH value for conidial germination was 3.86-9.23. The conidial could germinate quickly in saturated humidity or water drops and could not germinate when the relative humidity is less than 81%. The lethal temperature of the conidia was 55℃ for 10 min. When A. obpyriformis grew in light and darkness alternatively it could produce spores normally. But A. obpyriformis cultured in continuous light and darkness would result in slower growth and a few spores generation. A. obpyriformis could grow and generate spores in the medium containing aloe and make use of various monosaccharides, polysaccharides and alcohols as carbon souce as well as L-leucine and ammonium sulfate as nitrogen source.

Key words: Aloe. L, Black spot, Alternaria obpyriformis T Y Zhang, Morphology identification, Culture characteristic

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Cite this article

LU Hong-xue, ZHAO Ming-min, LI Jian-qiang. Studies on Identification and Culture Characteristic of Alternaria obpyriformis T Y Zhang[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2007, 22(1): 156-160. doi: 10.3321/j.issn:1000-7091.2007.01.037.

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