Location of Spontaneously Deleted Region of Wheat Yellow Mosaic Virus RNA2 in Inoculated Wheat

doi:10.3321/j.issn:1000-7091.2006.01.019

Abstract

Abstract: Fungus-transmitted plant viruses are liable to naturally delete some internal sequences from their genomes in infected plants during repeated mechanical passages.In this paper,a deleted mutation in RNA2 of WYMV was detected after the 27 passage of mechanical innoculation in wheat by RT-PCR and Northern blot.Sequence analysis showed that a contiguous sequence of 2595 nucleotides(from nt 214 to nt 2808) was internally deleted from WYMV RNA2 and flanked by seven reverse repeated compatible nucleotides which can form a loop-stem structure.A short sequence rich of base A(or U) connect to the left deleted region,which is the deletion signal in many viruses.The possible mechanism of the generation of deleted RNA2 of WYMV is discussed.

Key words: WYMV, Repeated mechanical passages, Location of spontaneously of RNA2

CLC Number:

S432.41

ZHANG Wei-hua, YANG Jun, HAN Cheng-gui, LI Da-wei, YU Jia-lin. Location of Spontaneously Deleted Region of Wheat Yellow Mosaic Virus RNA2 in Inoculated Wheat[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2006, 21(1): 80-85. doi: 10.3321/j.issn:1000-7091.2006.01.019.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.3321/j.issn:1000-7091.2006.01.019

http://www.hbnxb.net/EN/Y2006/V21/I1/80

References

[1] 王鸣歧,刘国士,陆秀梅.小麦梭斑花叶病毒在我国发生的初步研究[J].四川农业科技,1980,(1):34-35.
[2] 陶家风,秦家忠,肖际亨,等.四川土传小麦黄花叶病的研究[J].植物病理学报,1980,10(1):5-25.
[3] 于善谦,陈仲宜,徐来升,等.发生在我国的小麦黄花叶病[J].植物保护学报,1986,13(4):217-219.
[4] Abe H,Tamada T.Parasite of polymyxa betae[J].Ann Phytopath Soc Japan,1986,52:35-247.
[5] 于嘉林,晏立英,苏宁,等.小麦黄花叶病毒(WYMV)基因组核苷酸序列分析[J].中国科学(C辑),1999,29(6):639-644
[6] Tamada T,Schmitt C,Saito M,et al.High resolution analysis of the readthrough domain of beet necrotic yellow vein virus readthrough protein:a KTER motif is important for efficient transmission of the virus by polymyxa betae [J].Journal of General Virology,1996,77,1359-1367
[7] 陈剑平.中国禾谷多黏菌传麦类病毒研究现状与展望[J].自然科学进展,2005,15(5):524-531.
[8] Schenk P,Sohn A,Adams M J,et al.Movement of Bymoviruses and functions of RNA2-encoded proteins of Barleyyellow mosaic virus[J].Agronomie,1995,15:387-391.
[9] 杨军,张卫华,尚巧霞,等.小麦黄色花叶病毒低分子量RNA1的鉴定[J].中国病毒学2005,20(2):179-183.
[10] Domingo E,Holland J J.RNA virus mutation and fitness for survival [J].Annu Rev Microbiol,1997,51:151-178.
[11] Nagy P D,Simon A E.New insights into the mechanisms of RNA recombination[J].Virology,1997,235:1-9.
[12] Pogany J,Romero J,Huang Q,et al,De novo generation of defective interfering-like RNAs in broad bean mottle bromovirus[J].Virol,1995,212:574-586.
[13] Damayanti T A,Nageno H,Mise K,et al.brome mosaic virus defective RNAs generated during infection of barley plants[J].J Gen Virol,1999,80:2511-2518.
[14] Chen J P.Effect of cultivation temperature on the spontaneous development of deletion in soil-borne wheat mosaic furovirus RNA2[J].Molecular Plant Pathology,1995,85(3):299-306.
[15] Nagy P D,Bujarski J J.Targeting the site of RNA-RNA tecombination in brome mosaic virus with antisense sequences[J].Proc Natl Acad Sci USA,1993,90:6390-6394.
[16] White K A,Morris T J.Nonhomologous RNA recombination in tombusviruses:generation and evolution of defective interfering RNAs by stepwise deletions[J].J Virol,1994,68:14
[17] Wu W,Blumberg B M,Fay P J,et al.Strand transfer mediated by human immunodeficiency virus RT in vitro is promoted by pausing and results in misincorporation[J].J Biol Chem,1995,270:325-332.
[18] Nagy P D,Bujarski J J.Engineering of homologous recombination hotspots with AU-rich sequences in brome mosaic virus[J].J Virol,1997,71:3799-3810.
[19] Pilipenko E V,Gmyl A P,Agol V I.A model for rearrangements in RNA genomes[J].Nucleic Acids Res,1995,23:1870-1875.
[20] Knorr D A,Mullin R H,Hearne P Q,et al.De novo generation of defective interfering RNAs of tomato bushy stunt virus by high multiplicity passage [J].Virology Mar,1991,181(1):193-202.

Please choose a citation manager

Content to export