Abstract:
In single-enzyme RT-PCR,Taq enzyme plays,roles of both reverse-transcripise and DNA polymerise.The reaction conditions of single-enzyme RT-PCR was researched in this paper in order to detect PSTV rapidly.The results indicated that when the anneal temperature was 37℃ and the circles were more than 10 times in reverse-transcription phase,a relative band of PSTV was obtained by PCR which could be proved using nested-set PCR.Single-enzyme RT-PCR was simple and low-cost,so it could play on important role for the detection of PSTV.
Key words:
PSTV,
Single-enzyme RT-PCR,
Taqenzyme
CLC Number:
Ye Ming, Sheng Changzhong, Shi Ming, An Chunju, Ji Junli, Wu Xuefeng, Du Rongqian, Li Desen. Detection of Potato Spindl a Tuber Viroid by Single- enzyme RT-PCR[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2002, 17(1): 41-45. doi: 10.3321/j.issn:1000-7091.2002.01.009.