Abstract:
Hypericin has been reported as the active compound found in Hypericum perforatum.Studies have shown that Hypericin has the abilities of anti-depression,anti-Bacteria anti-virus,anti-cancer and so on.But the content of Hypericin of Hypericum perforatum is not enough in natural condition,the extraction of Hypericin was not match the need of applications.To study the synthesis approach and conversion in vitro of Hypericin,the full length cDNA of Hypericin Synthase(Hyp)was amplified from the RNA of Hypericum perforatum by RT-PCR and cloned into the plasmid pET30a to achieve the expression vector pET30-Hyp.Hypericin Synthase(Hyp) could be expressed in E.coli BL21(DE3)after IPTG induction.Hyp was purified from the supernatant of the bacterial lysate by Ni-NTA his bind resin.A protein band with predicted size about 20 KD was detected by SDS_PAGE.
Key words:
Hypericin,
Hypericum perforatum,
pET30a
CLC Number:
WANG Li, ZHANG Na, ZHANG Xiu-qing, SUN Jun-she. Cloning and Expression of Hypericin Synthase[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2005, 20(4): 1-3. doi: 10.3321/j.issn:1000-7091.2005.04.001.