Abstract:
Rye chromatins of three derivatives from Triticale×Triticum aestivum were shown by fluorescent in situ hybridization using total genomic DNA as aprobe. Fluorescent in situ hybridization indicated all of the three lines tested here carried a translocation between 1RS and a wheat chromosome arm. The breakpoints were at or closely near to the centromere. Hybridization signals are evidently present during all stages of mitotic cell cycle. 1RS chromosome arms was discernible as domains through nucleus at interphase. C-banding reveals the three translocation lines are 1BL/1RS translocations.
Key words:
Fluorescent in situ hybridization,
Mitosis,
Wheat rye translocation,
C-banding
Shi Yunsu, Li Hongjie, Guo Beihai, Zhang Yanmin, Wang Zining, Wen Zhiyu. Identification of 1B/1R Translocations in Wheat Background by Fluorescent in Situ Hybridization[J]. ACTA AGRICULTURAE BOREALI-SINICA, 1999, 14(1): 18-21. doi: 10.3321/j.issn:1000-7091.1999.01.004.