Effects of Short-term Submergence on Growth of Rice Seedlings and Activities of Key Nitrogen Metabolism Enzymes in Roots

doi:10.7668/hbnxb.20191627

Abstract

Abstract: This study was conducted to research the effects of short-term submergence on soil oxygen concentration and the effects of the biomass, plant height of rice seedlings and the activities of key enzymes of nitrogen metabolism in roots, and explore the physiological mechanism of short-term submergence on rice seedling growth and root nitrogen metabolism. The experiment was carried out under natural light condition in small arched shed and in artificial climate box, respectively. Five treatments were set:non submergence treatment (CK), submergence treatment from one-leaf and one-heart stage to two-leaf and one-heart stage (T1), submergence treatment from two-leaf and one-heart stage to three-leaf and one-heart stage (T3), submergence treatment from three-leaf and one-heart stage to four-leaf and one-heart stage (T3) and submergence treatment in whole process (T4). At the beginning and end of each submergence treatment, the rice seedling biomass, plant height and key enzyme activities of nitrogen metabolism in root were analyzed. The results showed that under flooding conditions, the oxygen concentration in the seedling soil dropped rapidly and approached zero after about 5 hours. After the flooding treatment was removed, with the loss of gravitational water in the soil, the oxygen concentration in the soil was less than 5 hours rapidly increase to atmospheric oxygen concentration state; no matter from which leaf age, the short-term submergence treatment promoted the growth of rice seedlings in a short time, and the fresh weight of shoot and underground root increased compared with the control. The effects of short-term submergence on the activities of key enzymes of nitrogen metabolism were basically the same at different leaf ages, and in most cases, the activities of various nitrogen metabolism enzymes were decreased. The effects of short-term submergence on rice seedlings in different leaf ages were basically the same. However, the effects of short-term submergence before three leaf stage on the activities of nitrogen metabolism enzymes in rice seedling roots were different from those at and after tillering stage.

Key words: Rice, Short-term submergence, Hypoxia stress, Nitrogen metabolism, Enzyme activity

CLC Number:

CHEN Jing, WANG Chaoyun, YI Yongjian, WANG Hongying, YI Zhenxie, ZHOU Wanlai. Effects of Short-term Submergence on Growth of Rice Seedlings and Activities of Key Nitrogen Metabolism Enzymes in Roots[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(2): 99-107. doi: 10.7668/hbnxb.20191627.

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References

[1] 胡志华, 朱练峰, 林育炯, 胡继杰, 张均华, 金千瑜. 水稻氮代谢对根际氧气供应的响应研究[J]. 中国稻米, 2015,21(1):12-17. doi:10.3969/j.issn.1006-8082.2015.01.003.
Hu Z H, Zhu L F, Lin Y J, Hu J J, Zhang J H, Jin Q Y. Research on rice nitrogen metabolism response to oxygen content in rhizosphere[J]. China Rice, 2015,21(1):12-17.
[2] 赵霞, 杜朝云, 徐春梅, 杨华伟, 吕泽林, 章秀福. 水稻对低氧环境的适应及其机制研究进展[J]. 作物杂志, 2015(3):5-12. doi:10.16035/j.issn.1001-7283.2015.03.002.
Zhao X, Du C Y, Xu C M, Yang H W, Lü Z L, Zhang X F. Review of acclimating mechanism tohypoxia condition in rice[J]. Crops, 2015(3):5-12.
[3] 徐春梅, 陈丽萍, 王丹英, 陈松, 章秀福, 石庆华. 低氧胁迫对水稻幼苗根系功能和氮代谢相关酶活性的影响[J]. 中国农业科学, 2016,49(8):1625-1634. doi:10.3864/j.issn.0578-1752.2016.08.020.
Xu C M, Chen L P, Wang D Y, Chen S, Zhang X F, Shi Q H. Effects of low oxygen stress on the root function and enzyme activities related to nitrogen metabolism in roots of rice seedlings[J]. Scientia Agricultura Sinica, 2016,49(8):1625-1634.
[4] 郭世荣. 营养液溶氧浓度对黄瓜和番茄根系呼吸强度的影响[J]. 园艺学报, 2000,27(2):141-142.doi:10.3321/j.issn:0513-353X.2000.02.016.
Guo S R. Effect of dissolved oxygen concentrations in nutrient solution on the respiratory intensity of cucumber and tomato roots[J]. Acta Horticulturae Sinica, 2000,27(2):141-142.
[5] 生利霞, 束怀瑞. 低氧胁迫对平邑甜茶根系活力及氮代谢相关酶活性的影响[J]. 园艺学报, 2008,35(1):7-12.doi:10.3321/j.issn:0513-353X.2008.01.002.
Sheng L X, Shu H R. Effects of hypoxia on the root activity, respiratory rate and the activities of enzymes involved in nitrogen metabolism in roots of Malus hupehensis Rehd[J]. Acta Horticulturae Sinica,2008,35(1):7-12.
[6] 刘义玲, 李天来, 孙周平, 陈亚东. 根际低氧胁迫对网纹甜瓜生长、根呼吸代谢及抗氧化酶活性的影响[J]. 应用生态学报, 2010,21(6):1439-1445. doi:10.13287/j.1001-9332.2010.0227.
Liu Y L,Li T L,Sun Z P,Chen Y D. Impacts of root-zone hypoxia stress on muskmelon growth, its root respiratory metabolism,and antioxidative enzyme activities[J]. Chinese Journal of Applied Ecology, 2010,21(6):1439-1445.
[7] 生利霞, 巴金磊, 冯立国, 王猛, 束怀瑞. 低氧胁迫对樱桃根系呼吸功能及氮代谢的影响[J]. 内蒙古农业大学学报(自然科学版), 2012,33(S1):23-27. doi:10.16853/j.cnki.1009-3575.2012.z1.006.
Sheng L X, Ba J L, Feng L G, Wang M, Shu H R. Effects of hypoxia on the root activity,respiratory rate and the nitrogen metabolism in roots of cherry[J]. Journal of Inner Mongolia Agricultural University(Natural Science Edition),2012,33(S1):23-27.
[8] 周勃, 郁敏, 米银法. 根际低氧对不同抗性牡丹植株呼吸代谢的影响[J]. 河南农业大学学报, 2016,50(6):734-738,747. doi:10.16445/j.cnki.1000-2340.2016.06.005.
Zhou B, Yu M, Mi Y F. Effect of root zone hypoxia stress on respiratory metabolism between two Paeonia suffruticosa varieties[J]. Journal of Henan Agricultural University, 2016,50(6):734-738,747.
[9] 刘晓慧, 伍海兵, 张圣美, 尚静, 张爱冬, 朱宗文, 田守波, 吴雪霞. 淹水胁迫对丝瓜幼苗生长及呼吸酶活性的影响[J]. 江西农业学报, 2020,32(3):48-54. doi:10.19386/j.cnki.jxnyxb.2020.03.09.
Liu X H, Wu H B, Zhang S M, Shang J, Zhang A D, Zhu Z W, Tian S B, Wu X X. Effects of waterlogging stress on growth and respiratory enzyme activities of luffa seedlings[J]. Acta Agriculturae Jiangxi, 2020,32(3):48-54.
[10] Diab H, Limami A M. Reconfiguration of N metabolism upon hypoxia stress and recovery:roles of alanine aminotransferase (AlaAT) and glutamate dehydrogenase (GDH)[J]. Plants, 2016,5(2):25. doi:10.3390/plants5020025.
[11] Limami A M.Adaptations of nitrogen metabolism to oxygen deprivation in plants[M]//Low-oxygen stress in plants.Dongen, Francesco Licausi:Springer Vienna, 2014.
[12] Limami A M, Diab H, Lothier J. Nitrogen metabolism in plants under low oxygen stress[J]. Planta, 2014, 239(3):531-541. doi:10.1007/s00425-013-2015-9.
[13] 王荣栋, 尹经章, 作物栽培学[M]. 2版.北京:高等教育出版社, 2015.
Wang R D, Yin J Z. Crop cultivation[M]. 2ed.Beijing:Higher Education Press, 2015.
[14] 蒋彭炎, 马跃芳, 洪晓富, 冯来定, 史齐林, 顾宏辉. 水稻分蘖芽的环境敏感期研究[J]. 作物学报, 1994,20(3):290-296.
Jiang P Y, Ma Y F, Hong X F, Feng L D, Shi J L, Gu H H. Studies on the sensitive stage to environment during differentiation and development of tiller buds in rice plant[J]. Acta Agronomic Sinica, 1994,20(3):290-296.
[15] 马丽峰, 李佐同, 杨克军, 赵长江, 徐晶宇, 杨荣斌, 于高波, 赵莹, 石新新, 许艳梅, 刘承, 闫博巍. 没顶淹水对敏感性水稻幼苗生长及抗氧化酶活性的影响[J]. 植物生理学报, 2015,51(7):1082-1090. doi:10.13592/j.cnki.ppj.2015.0029.
Ma L F,Li Z T,Yang K J,Zhao C J,Xu J Y,Yang R B, Yu G B,Zhao Y,Shi X X,Xu Y M,Liu C,Yan B W. Effects of submergence on growth and antioxidant enzymes activities of sensitive rice (Oryza sativa) seedlings[J]. Plant Physiology Communications,2015,51(7):1082-1090.
[16] 武辉, 向镜, 陈惠哲, 张玉屏, 张义凯, 朱德峰. 外源调节剂对淹涝水稻幼苗株高及碳水化合物消耗的影响[J]. 应用生态学报, 2018,29(1):149-157. doi:10.13287/j.1001-9332.201801.021.
Wu H, Xiang J, Chen H Z, Zhang Y P,Zhang Y K, Zhu D F.Effects of exogenous growth regulators on plant elongation and carbohydrate consumption of rice seedlings under submergence[J]. Chinese Journal of Applied Ecology, 2018,29(1):149-157.
[17] 向镜, 陈惠哲, 张玉屏, 张义凯, 朱德峰. 淹涝条件下水温对水稻幼苗形态和生理的影响[J]. 中国水稻科学, 2016,30(5):525-531. doi:10.16819/j.1001-7216.2016.5183.
Xiang J, Chen H Z, Zhang Y P, Zhang Y K, Zhu D F.Morphological and physiological responses of rice seedlings to water temperature under complete submergence[J]. Chinese Rice Sciences, 2016,30(5):525-531.
[18] 王矿, 王友贞, 汤广民. 分蘖期水稻对淹水胁迫的响应规律研究[J]. 灌溉排水学报, 2014,33(6):58-60, 91. doi:10.13522/j.cnki.ggps.2014.06.013.
Wang K, Wang Y Z, Tang G M. Response of rice to waterlogging stress in tillering stage[J]. Journal of Irrigation and Drainage, 2014,33(6):58-60,91.
[19] Kuroha T, Ashikari M. Molecular mechanisms and future improvement of submergence tolerance in rice[J]. Molecular Breeding, 2020,40(4):1-4.doi:10.1007/s11032-020-01122-y.
[20] Kende H, van der Knaap E, Cho H T.Deepwater rice:a model plant to study stem elongation[J].Plant Physiology,1998,118(4):1105-1110.doi:10.1104/pp.118.4.1105.
[21] Su P H, Lin C H. Metabolic responses of luffa roots to long-term flooding[J]. Journal of Plant Physiology, 1996, 148(6):735-740.doi:10.1016/S0176-1617(96) 80376-2.
[22] 张晓果, 王丹英, 计成林, 徐春梅, 陈松, 章秀福. 水稻氮素吸收利用研究进展[J]. 中国稻米, 2015,21(5):13-19. doi:10.3969/j.issn.1006-8082.2015.05.003.
Zhang X G,Wang D Y,Ji C L,Xu C M,Chen S,Zhang X F.Nitrogen absorption and utilization on rice[J]. China Rice, 2015, 21(5):13-19.
[23] Appenroth K J, Meco R, Jourdan V, Lillo C. Phytochrome and post-translational regulation of nitrate reductase in higher plants[J]. Plant Science, 2000,159(1):51-56. doi:10.1016/S0168-9452(00) 00323-X.

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