Effect of Fertilizer Types and Fertilization Methods on 2-acetyl-1-pyrroline Content in Aromatic Rice

doi:10.7668/hbnxb.2016.05.023

Abstract

Abstract: In order to explore the impact of fertilizer types and fertilization methods on 2-acetyl-1-pyrroline(2AP) content in aromatic rice and its physiological characters,the widely used and popular aromatic rice cultivar,Yuxiangyouzhan was used as material in the field trials.The experiment consisted of two fertilization methods i.e.deep application of fertilizer and manual application of fertilizer,and two fertilizer types i.e.the chemical fertilizer ammonium bicarbonate+calciumsuperphosphate+potassium chloride and the compound fertilizer,the nutrient(NPK) content in each treatment with equal quality.The 2AP content in mature grain as well as the proline content,proline dehydrogenase activity,pyrroline-5-carboxylate synthetase activity,ornithine aminotransferase activity and pyrroline-5-carboxylate acid content in leaves at tillering stage,booting stage,flowering stage and maturity were measured.The results showed that fertilizer types,fertilization methods and their interaction significantly affected the 2AP content in mature grains.The manual application of ammonium bicarbonate+calciumsuperphosphate+potassium chloride had the highest 2AP content in mature grains(59.86 ng/g). And the 2AP content in mature grains was in positive correlation with pyrroline-5-carboxylate acid content in leaves at maturity significantly(r=0.957 1^*).The results provide theoretical reference for the development of scented rice fertilization technology.

Key words: Fertilizers type, Fertilization methods, Aromatic rice, 2-acetyl-1-pyrroline, Physiological basis

CLC Number:

S511
S223

MO Zhaowen, FAN Pingshan, PAN Shenggang, WANG Zaiman, TIAN Hua, DUAN Meiyang, TANG Xiangru. Effect of Fertilizer Types and Fertilization Methods on 2-acetyl-1-pyrroline Content in Aromatic Rice[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2016, 31(5): 152-158. doi: 10.7668/hbnxb.2016.05.023.

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References

[1] Schieberle P.The role of free amino acids present in yeast as precursors of the odorants 2-acetyl-1-pyrroline and 2-acetyltetrahydropyridine in wheat bread crust[J].ZeitschriftfürLebensmittel-Untersuchung und Forschung,1990,191(3):206-209.
[2] Hashemi F G,Rafii M Y,Ismail M R,et al.Biochemical,genetic and molecular advances of fragrance characteristics in rice[J].Critical Reviews in Plant Sciences,2013,32(6):445-457.
[3] Kovach M J,Calingacion M N,Fitzgerald M A,et al.The origin and evolution of fragrance in rice(Oryza sativa L.)[J].Proceedings of the National Academy of Sciences of the United States of America,2009,106(34):14444-14449.
[4] 叶兴锋,张伟,周屹峰,等.水稻籽粒香味性状研究进展[J].现代农业科技,2013(6):9,16.
[5] 黄忠林,唐湘如,王玉良,等.增香栽培对香稻香气和产量的影响及其相关生理机制[J].中国农业科学,2012,45(6):1054-1065.
[6] 冯梦诗,叶俊,侯莉莉,等.无选择标记基因的优质香稻新品系培育[J].华北农学报,2015,30(5):92-96.
[7] 张江丽,李苏洁,普玉娇,等.不同来源水稻种质资源香味基因badh2位点的鉴定[J].分子植物育种,2015,13(4):727-733.
[8] 姜达,卢小勇,王延春,等.27种香稻品种badh2突变位点序列的分析[J].分子植物育种,2015,13(2):276-280.
[9] 余亚莹,邵高能,圣忠华,等.国内外香稻资源遗传多样性研究[J].植物分类与资源学报,2015,37(6):871-880.
[10] Bradbury L T,Fitzgerald T L,Henry R J,et al.The gene for fragrance in rice[J].Plant Biotechnology Journal,2005,3(3):363-370.
[11] Fitzgerald M A,Hamilton N,Verhoeven H A,et al.Is there a second fragrance gene in rice?[J].Plant Biotechnology Journal,2008,6(4):416-423.
[12] Chen S,Yang Y,Shi W,et al.Badh2,encoding betaine aldehyde dehydrogenase,inhibits the biosynthesis of 2-acetyl-1-pyrroline,a major component in rice fragrance[J].The Plant Cell,2008,20(7):1850-1861.
[13] Sakthivel K,Sundaram R M,Shobha R N,et al.Genetic and molecular basis of fragrance in rice[J].Biotechnology Advances,2009,27(4):468-473.
[14] Boling A A,Bouman B,Tuong T P,et al.Yield gap analysis and the effect of nitrogen and water on photoperiod-sensitive Jasmine rice in north-east Thailand[J].NJAS-Wageningen Journal of Life Sciences,2011,58(1/2):11-19.
[15] Shao G,Tang S,Chen M,et al.Haplotype variation at Badh2,the gene determining fragrance in rice[J].Genomics,2013,101(2):157-162.
[16] Shi Y Q,Zhao G C,Xu X L,et al.Discovery of a new fragrance allele and development of functional markers for identifying diverse fragrant genotypes in rice[J].Molecular Breeding,2014,33(3):701-708.
[17] Vijayalakshmi P,Vishnukiran T,Kumari B R,et al.Biochemical and physiological characterization for nitrogen use efficiency in aromatic rice genotypes[J].Field Crops Research,2015,179:132-143.
[18] Shan Q W,Zhang Y,Chen K L,et al.Creation of fragrant rice by targeted knockout of the OsBADH2 gene using TALEN technology[J].Plant Biotechnology Journal,2015,13(6):791-800.
[19] Buttery R G,Ling L C,Juliano B O,et al.Cooked rice aroma and 2-acetyl-1-pyrroline[J].Journal of Agricultural and Food Chemistry,1983,31(4):823-826.
[20] Buttery R G,Ling L C,Mon T R.Quantitative analysis of 2-acetyl-1-pyrroline in rice[J].Journal of Agricultural and Food Chemistry,1986,34(1):112-114.
[21] Buttery R G,Turnbaugh J G,Ling L C.Contribution of volatiles to rice aroma[J].Journal of Agricultural and Food Chemistry,1988,36(5):1006-1009.
[22] Paule C M,Powers J J.Sensory and chemical examination of aromatic and nonaromatic rices[J].Journal of Food Science,1989,54(2):343-346.
[23] Bergman C J,Delgado J T,Bryant R,et al.Rapid gas chromatographic technique for quantifying 2-acetyl-1-pyrroline and hexanal in rice(Oryza sativa L.)[J].Cereal Chemistry,2000,77(4):454-458.
[24] Jezussek M,Juliano B O,Schieberle P.Comparison of key aroma compounds in cooked brown rice varieties based on aroma extract dilution analyses[J].Journal of Agricultural and Food Chemistry,2002,50(5):1101-1105.
[25] Sriseadka T,Wongpornchai S,Kitsawatpaiboon P.Rapid method for quantitative analysis of the aroma impact compound,2-acetyl-1-pyrroline,in fragrant rice using automated headspace gas chromatography[J].Journal of Agricultural and Food Chemistry,2006,54(21):8183-8189.
[26] Kumar S G,Reddy A M,Sudhakar C.NaCl effects on proline metabolism in two high yielding genotypes of mulberry(Morus alba L.)with contrasting salt tolerance[J].Plant Science,2003,165(6):1245-1251.
[27] Huang T C,Huang Y W,Hung H J,et al.Delta(1)-Pyrroline-5-carboxylic acid formed by proline dehydrogenase from the Bacillus subtilis ssp natto expressed in Escherichia coli as a precursor for 2-acetyl-1-pyrroline[J].Journal of Agricultural and Food Chemistry,2007,55(13):5097-5102.
[28] Huang T C,Teng C S,Chang J L,et al.Biosynthetic mechanism of 2-acetyl-1-pyrroline and its relationship with Delta(1)-pyrroline-5-carboxylic acid and methylglyoxal in aromatic rice(Oryza sativa L.)callus[J].Journal of Agricultural and Food Chemistry,2008,56(16):7399-7404.
[29] Wakte K V,Kad T D,Zanan R L,et al.Mechanism of 2-acetyl-1-pyrroline biosynthesis in Bassia latifolia Roxb.flowers[J].Physiology and Molecular Biology of Plants,2011,17(3):231-237.
[30] Kaikavoosi K,Kad T D,Zanan R L,et al.2-Acetyl-1-Pyrroline augmentation in scented indica rice(Oryza sativa L.) varieties through delta(1)-Pyrroline-5-Carboxylate synthetase(P5CS) gene transformation[J].Applied Biochemistry and Biotechnology,2015,177(7):1466-1479.
[31] Goufo P,Duan M,Wongpornchai S,et al.Some factors affecting the concentration of the aroma compound 2-acetyl-1-pyrroline in two fragrant rice cultivars grown in South China[J].Frontiers of Agriculture in China,2010,4(1):1-9.
[32] 阳树英,邹应斌,夏冰,等.中国传统地方香稻品种资源的多样性及其在特殊生境成香机理的探讨[J].中国稻米,2015,21(3):1-7.
[33] Bhattacharjee P,Singhal R S,Kulkarni P R.Basmati rice:a review[J].International Journal of Food Science & Technology,2002,37(1):1-12.
[34] 阳树英,邹应斌,夏冰,等.湖南主要香稻产区气候生态因子对水稻香气质量的影响[J].中国稻米,2013,19(4):44-49.
[35] 杨晓娟,唐湘如,闻祥成,等.播种期对早季香稻香气形成,品质及产量的影响[J].华北农学报,2014,29(3):128-135.
[36] 田卡,唐湘如,段美洋,等.灌浆成熟期水分对香稻香气及生理特性的影响[J].灌溉排水学报,2010,29(6):91-93.
[37] 王培,唐湘如,田华,等.孕穗期水分灌溉处理对香稻香气及生理特性的影响[J].广东农业科学,2013,40(8):1-3.
[38] 王培,肖立中,唐湘如,等.分蘖期水分对香稻香气及生理特性的影响[J].灌溉排水学报,2013,32(1):103-105.
[39] 李艳红,唐湘如,潘圣刚,等.分蘖期水氮互作对香稻香气、产量及稻米品质的影响[J].华北农学报,2014,29(1):159-164.
[40] 孙树侠,刘书城.水稻的香味及N,Zn肥对香味效应的研究[J].作物学报,1991,17(6):430-435.
[41] Itani T,Tamaki M,Hayata Y,et al.Variation of 2-acetyl-1-pyrroline concentration in aromatic rice grains collected in the same region in Japan and factors affecting its concentration[J].Plant Production Science,2004,7(2):178-183.
[42] Yang S Y,Zou Y B,Liang Y Z,et al.Role of soil total Nitrogen in aroma synthesis of traditional regional aromatic rice in China[J].Field Crops Research,2012,125(1):151-160.
[43] 罗一鸣,肖立中,潘圣刚,等.钾肥对香稻香气及稻米品质的影响[J].西南农业学报,2014,27(3):1147-1153.
[44] 黄淑贞.湖南香稻产地土壤特性与稻米品质的关系[J].湖南农业科学,1990,4:37-40.
[45] 胡树林,徐庆国,黄启为.香米品质与微量元素含量特征关系的研究[J].作物研究,2001,15(4):12-15.
[46] 胡树林,黄启为,徐庆国.不同产地香米微量元素含量差异及吸收富集特征研究[J].作物研究,2002,16(1):14-16.
[47] 唐湘如,吴密.施用锌、铁、镧肥对香稻糙米香气和剑叶脯氨酸含量的影响[J].杂交水稻,2006,21(6):69-72.
[48] 黄锦霞,肖迪,唐湘如.施锌对香稻产量、香气和品质的影响[J].耕作与栽培,2010(3):5-7.
[49] 黄锦霞,肖迪,段美洋,等.不同锌肥施用量对香稻产量和糙米香气含量的影响[J].华北农学报,2008,23(S2):290-292.
[50] 肖迪,黄锦霞,唐湘如,等.镧对香稻糙米香气的影响及其机理研究[J].西南农业学报,2009,22(2):364-367.
[51] 肖迪,黄锦霞,段美洋,等.不同镧肥施用量对香稻产量及糙米香气含量的影响[J].华南农业大学学报,2010,31(1):115-116.
[52] Mo Z,Li W,Pan S,et al.Shading during the grain filling period increases 2-acetyl-1-pyrroline content in fragrant rice[J].Rice,2015,8(1):9.
[53] 段美洋,黎国喜,田华,等.增香剂对香稻香气和生理特性的影响[J].华南农业大学学报,2009,30(3):1-3.
[54] Goufo P,Wongpornchai S,Tang X R.Decrease in rice aroma after application of growth regulators[J].Agronomy for Sustainable Development,2011,31(2):349-359.
[55] 王抄抄,孔雷蕾,李妹娟,等.6-苄氨基腺嘌呤(6-BA)对香稻香气2-乙酰-1-吡咯啉(2AP)的影响[J].西南农业学报,2015,28(3):1033-1037.
[56] Bates L S,Waldren R P,Teare I D.Rapid determination of free proline for water stress studies[J].Plant and Soil,1973,39(1):205-207.
[57] Ncube B,Finnie J F,Van Staden J.Dissecting the stress metabolic alterations in in vitro Cyrtanthusregenerants[J].Plant Physiology and Biochemistry,2013,65(6):102-110.
[58] Sánchez E,Ruiz J M,Romero L.Proline metabolism in response to nitrogen toxicity in fruit of french bean plants(Phaseolus vulgari s L.cv Strike)[J].Scientia Horticulturae,2002,93(3/4):225-233.
[59] Chen C T,Chen L M,Lin C C,et al.Regulation of proline accumulation in detached rice leaves exposed to excess copper[J].Plant Science,2001,160(2):283-290.
[60] Wu M L,Chou K L,Wu C R,et al.Characterization and the possible formation mechanism of 2-acetyl-1-pyrroline in aromatic vegetable soybean(Glycine max L.)[J].Journal of Food Science,2009,74(5):192-197.
[61] Yoshikawa K,Libbey L M,Cobb W Y,et al.1-Pyrroline:the odor component of strecker-degraded proline and ornithine[J].Journal of Food Science,1965,30(6):991-994.
[62] Seitz L M,Wright R L,Waniska R D,et al.Contribution of 2-acetyl-1-pyrroline to odors from wetted ground pearl millet[J].Journal of Agricultural and Food Chemistry,1993,41(6):955-958.
[63] Thimmaraju R,Bhagyalakshmi N,Narayan M S,et al. In vitro culture of Pandanus amaryllifolius and enhancement of 2-acetyl-1-pyrroline,the major flavouring compound of aromatic rice,by precursor feeding of L-proline[J].Journal of the Science of Food and Agriculture,2005,85(15):2527-2534.
[64] Yoshihashi T,Huong N T,Inatomi H.Precursors of 2-acetyl-1-pyrroline,a potent flavor compound of an aromatic rice variety[J].Journal of Agricultural and Food Chemistry,2002,50(7):2001-2004.
[65] 赵贵林,陈强,胡国霞,等.水稻脯氨酸代谢关键酶对水分胁迫的响应[J].干旱地区农业研究,2011,29(3):80-83.

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