Abstract: In order to established confectionary sunflower molecular markers reaction system, the study assuming DNA template from four-leaf stage of confectionary sunflowers leaves for experimental materials, this study used the single factor test and orthogonal design for optimizing six SSR-PCR reaction system factors (10×PCR Buffer, Mg²⁺, dNTPs, primers, Taq DNA polymerase, DNA template and compared different concentrations of Mg²⁺, dNTPs, Taq DNA polymerase and DNA template amplification effect of the impact in SSR-PCR reaction system.The result showed that the range of the influence of six factors in the reaction system was Mg²⁺> Taq DNA polymerase (primer)>DNA template>10×PCR Buffer>dNTPs.And this study established confectionary sunflower SSR-PCR reaction system(20 μL):10×PCR Buffer 0.2 mmol/L, Mg²⁺ 2.0 mmol/L, dNTPs 1.8 mmol/L, Taq DNA polymerase 0.2 U, DNA 50 ng, primer 1.5 mmol/L.

Key words: Confectionary sunflower, SSR-PCR, Reaction system

CLC Number: 

• S565.5