Effects of Phospholipase C Inhibitor U-73122 on Conidial Germination and HT-toxin Activity of Setosphaeria turcica

doi:10.7668/hbnxb.2010.06.022

Abstract

Abstract: Phospholipase C(PLC)could catalyze the hydrolysis of phospholipid molecules on cell membrane and generate two second messenger molecules IP3 and DAG, which activated Ca“signal pathway and protein kinase C re— spectively.It had been proved that PLC modulated pathogenicity and development of plant pathogenic fungi.In this research, U-73 1 22, the specific inhibitor of PLCs, was used to study functions of PLCs in Setosphaeria turcica prelimina— ry.U一73122 showed significant inhibition effects on hyphal growth and conidial germination.The higher was the inhibitor concentration, the lower was the germination ratio of spores.U-73 122 caused the germ tube shortening and cell swelling irregularly.In addition, U一73 1 22 could also obviously decrease the biological activity of HT-toxin.It was indi— cated that PLC might regulate hyphal growth and conidial germination by controlling the cell polarity, and also involved in the biosynthesis of HT—toxin in Setosphaeria turcica.

Key words: Setosphaeria turcica, Phospholipase C, U·73 122, Conidia, HT-toxin

CLC Number:

S435.131

HAO Zhi-min, HAO Jie, LI Zhi-yong, DONG Jin-gao. Effects of Phospholipase C Inhibitor U-73122 on Conidial Germination and HT-toxin Activity of Setosphaeria turcica[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(6): 118-121. doi: 10.7668/hbnxb.2010.06.022.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.7668/hbnxb.2010.06.022

http://www.hbnxb.net/EN/Y2010/V25/I6/118

References

[1] Wang P,Pan X,Waugh M.Signal transduction cascades regulating fungal development and virulence[J].Microbiology Molecular Biology Review,2000,64:746-785.
[2] Warwar V,Dickman M B.Effects of calcium and calmodulin on spore germination and appressorium development in Colletotrichum trifolii[J].Apply of Environmental Microbiology,1996,62:74-79.
[3] Schmidt M,Evellin S,Weernink P A,et al.A new phospholipase-C-calcium signaling pathway mediated by cyclic AMP and a Rap GTPase[J].Nature Cell Biology,2001,3:1020-1024.
[4] 宋颖琦,杨谦.磷脂酶在植物逆境胁迫信号传导中的作用[J].哈尔滨工业大学学报,2006,38(2):243-246.
[5] 张兵,吴乔.磷脂酶C-γ功能研究进展[J].细胞生物学杂志,2004,26(2):93-96.
[6] 曹志艳,于清,范永山,等.玉米大斑病菌黑色素缺失突变体的获得及其生物学特性[J].植物保护学报,2007,34(3):268-272.
[7] Li S,Chen T,Zhu R.Effect of U-73122 on calcium channel activity in porcine myometrial cells and pancreatic beta-cell line RINm5F[J].Acta Pharmacologica Sinica,1997,18(5):385-390.
[8] Ramadurai S M,Chen W Y.Cell-specific and developmental expression of phospholipase C-gamma and diacylglycerol in fetal lung[J].American Journal of Physiology-Lung Cellular and Molecular Physiology,2003,284:808-816.
[9] 王立安,王源超,李昌文,等.Ca2+信号途径参与稻瘟病菌分生孢子萌发及附着胞形成的调控[J].菌物系统,2003,22(3):457-465.
[10] Olivera G,Daiana B S,Anthony G Y.Mutation and divergence of the phospholipase C gene in Neurospora crassa[J].Fungal Genetics and Biology,2007,44:242-249.
[11] Kimberly J G,Maureen J D,Carlos E S.Cell wall integrity is dependent on the PKC1 signal transduction pathway in Cryptococcus neoformans[J].Molecular Microbiology,2005,58(2):393-408.
[12] Mojca B,Mattc L,Nick D R.Cross-talk between cAMP and calcium signalling in Aspergillus niger[J].Molecular Microbiology,2005,56(1):268-281.
[13] Revital R,Haim S,Emma L.The Aspergillus nidulans pkcA gene is involved in polarized growth,morphogenesis and maintenance of cell wall integrity[J].Current Genetics,2007,51:321-329.

Please choose a citation manager

Content to export