The Effects of Starch-synthesizing Genes on Rice Quality

doi:10.7668/hbnxb.2009.02.012

Abstract

Abstract: Granule-bound starch synthase (GBSS),Starch branching enzyme 1 (SBE1) and Starch branching enzyme 3(SBE3) are three major enzymes involved in starch biosynthesis in rice endosperm. The granule-bound starch synthase was encoded by the Wx gene,and the starch branching enzymes were encoded by the Sbe1 and Sbe3 gene.And molecular markers for Sbe1,Sbe3 developed based on sequence diversities between the rice subspecies indica and japonica,together with PCR2AccⅠmarker for Wx gene were used to investigate the genotypes of 183 rice cultivars.The result showed that the developed molecular markers for Wx,Sbe1,Sbe3 could distinguish indica or japonica alleles at three loci.Among all the 183 rice cultivars,eight genotype combinations were observed at Sbe1,Sbe3 and Wx loci. In order to explore the genetic effects of three genes,ANOVA and multiple comparison analysis were conducted. The results showed that rice cultivars with different genotypes exhibited different phenotypes,including amylose content,gel consistency and some of RVA characteristics,and the significant differences were observed. And the genetic effects of indica and japonica alleles at three gene loci were different,Wx gene had significant effects on mainly rice quality traits,followed by Sbe3 and Sbe1. The genetic effects of Sbe1 and Sbe3 in different backgrounds (Wxa, Wxb)were different. Interactions existed among Wx, Sbe1, Sbe3,Interaction was generally detected between Wx and Sbe3.

Key words: Starch-synthesizing genes, Amylose content, Gel consistency, Starch RVA characteristi

CLC Number:

S11.03

CHEN Feng, SUN Gong-chen, YANG Ze-feng, ZHANG Shi-yong, ZHANG Hong-rui, YANG Ya-chun, YUAN Shou-jiang, ZHANG Zheng-qiu. The Effects of Starch-synthesizing Genes on Rice Quality[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2009, 24(2): 55-59. doi: 10.7668/hbnxb.2009.02.012.

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