Cloning and Sequence Analysis of 18S rRNA Gene from Laminaria japonica Gametophytes and Its Application as an Internal Standard

doi:10.7668/hbnxb.2009.01.012

Abstract

Abstract: 18S rRNfl gene sequence of Laminaria japonica was obtained by 18S rRNfl gene cloning and sequencing(CnBank accession number:EU293553).The phylogenetic analysis among 8 spieces andan outgro(Nostoc spPCC7120) were constructed based on 18S rRNfl gene sequences by neighborjoining(NT) method. Based ongenetic distance from L. japonica of Phaeophyta to Nostoc sp.PCC7120 of C}anophyta,Griffithsia japonicathe result,theof Rhocbphytaand Chlamydomonas reinhardtii of Chlorophyta,was 0. 819,0. 303 and 0. 219 in turn. While among chmmophyteslongest geneticshortest was 0.distance was 0. 201 from Laminaria japonica of Phaeophyta to Rhodomonas sp.CS24 of Cryptophyta,thethe103 from L. japonica to Heterosigma carterae of Xanthophyta. Using 18S rRNfl gene as internal standardsemi-quantitative RT-PCR result revealed the expression of lhcf6 gene increased with enhanced light intensity.

Key words: Laminaria.japonica, 18S rRNA gene, lhcf6, Semi-quantitative PCR

CLC Number:

S917.3

BI Yan-hui, ZOU Dan-yan, ZHOU Zhi-gang. Cloning and Sequence Analysis of 18S rRNA Gene from Laminaria japonica Gametophytes and Its Application as an Internal Standard[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2009, 24(1): 49-54. doi: 10.7668/hbnxb.2009.01.012.

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References

1 fang Q,Li Z,71iou G,et al. lmmunosuppressive activities of fucoidan from Laminaria japonica [J].Chinese Journal of O- ceanology Limnology,2003,21：324一328.
2 J.源.我国药用海藻名录及其应用[J].海洋药物， 1982,3：45一48.
3 Qian A,Wang X,Deng Y,et al. Heavy metal biosorption sites studies of Laminaria japonica [J].Marine Science Bulltion， 2005,7(1)：87一91.
4 Wang X，qu H, Cai Z, et al厂the research on the adsorption effect on metal ions by imrmbilized marine algae [J].Acta O- ceanologica Sinica,2006,25 (2)：153一158.
5 吴晓，杨明亮，黄晓兰等，海带多糖的抗辐射作用与脾细胞凋亡[J]武汉大学学报:医学版，2004,25 (3) 239一252.
6 曾呈奎，吴超元.海带养殖学[M].北京:科学出版社， 1962.
7 周志刚，吴超元.海带无性繁殖系的形成及抱子体诱导 [J].生物工程学报，1998,14:109一111.
8 周志刚，史西志，胡远皆，等.中国海区小同养殖品系海带与长还带之问的遗传关系[J].中国水产科学，2003, 10:474一480.
9 胡远皆，周志刚.海带抱子体DNA随即扩增反应条件优化[J].上海水产大学学报，2001,10:193一198.
10 史西志，毕燕会，周志刚.海带雄性配子体差异表达基因片段的克隆及筛选[J]1.水产学报，2005, 29： 666 - 669.
11 Voigt O, Eipenbeck D, Worheide G. Molecular evolution ofrDNA in early diverging Metawa：first comparative analysisand phogenetic application of complete SSC1 rRNA sec-ondary structures in Rrifera[J]BMC Evolutionary Biology，2008,8：69.
12 Stemien H K Slow molecular ewlution in 18S rDNA，rbdand nad5 genes of mosses compared with higher plants[J].Journal of Evolutionary Biology,2008,21：566一71.
13 Pace N R,Olsen GJ,Woese C R. Ribosomal RNA phoge-ny and the primary lines of evolutionary descent [J].Cell，1986,45：325一326.
14 Wheelis M L, Kandler O,Woese C R. On the nature of glob-al classification[J].Proceedings of the National Academy ofSciences of the United States of America,1992,89：2930-2934.
15 毕燕会，周志刚.海带配子体叶绿素a/ c结合蛋白基因lhcf6的序列特征与系统发生分析[J].高技术通thl,2008,18：84一90.
16 李爱芬，陈敏，周百成.褐藻光合作用色素一蛋白质复合物一一-于究进展和问题[J].植物学通报，1999,16：365一371.
17 毕燕会，周志刚.杂色藻类褐藻素一叶绿素结合蛋白的研究现状[J]上海水产大学学报，2007,16：177 -183.
18 Katoh T,1Vfimuro M，Takaichi S. Light-harvesting particlesisolated from a brown alga，Dictyota dlchotoma. Asuprarmlecular assembly of fucoxanthin-chlorophyll-proteincorrlexes[J].Biochimica ET Biopsica Acta,1989,976:233一240.
19 Escoubas J M,Lomas M,LaRoche J，et cd. Light intensityregulation of cab gene transcription is signaled by the redoxstate of the plastoquinone pool [J].Proceedings of the Na-tional Academy of Sciences of the United States of America，1995,92：10237一10241.
20 Pursiheirm S,Mulo P, Rintam aki E，et al. Coregulation oflight-harvesting complex II phosplao anon and Lhcb mRNAaccumulation in winter rye [J].Plant Journal,2001,26：317一327.
21 Provasoli L. Media and products for the cultivation ofmarinealgae[C]//Watanabe A, Hattori A. Culture and collectionsof algae.Japanese Society of Plant Physiology,To Japan，1968:63一75.
22 Ausubel F M,Brent R, Ingston R，et al. Clarrent Protocolsin Molecular Biology [M]. New York：J Wiley，lntersciencePress.1987：241一252.
23 ThompsonJ D,Iggins D G,Qbson TJ. CLUSTAI. W:Inrproving the sensitivity of progressive multiple sequence align-ment through sequence weighting，position specific gappenalties and weight matrix choice [J].Nucleic Acids Re-search,1994,22：4673一4680.
24 Kumar S,Tamara K,Takobsen I B,et ul. MEGA2：molecularevolutionary genetics analysis software [J].Bioinformatics，2001,17：1244一1245.
25 Centt E. Phycobilisomes[J].Annual Review Plant Physiolo-gy,1981,32：327一347.
26 Qazer A N. Light harvesting by phycobilisomes[J].AnnualReview Biochemistry,1985,14:47一77.
27 Creen B R. "Ihe chlorophyll-protein complexes of higherplant photosynthetic membranes,or just what green brand isX281that[J]Larkin PPhotosynthesis Research,1988,15：3一32
28 Folmar L C, Hemmer M Jing of estrogenic compounds using a，et ul. Expression profil-sheepshead minnow cD- NA macroarray[J].Environmental Health Perspectives， 2003,111：839一846.
29 Passaquet C,Lichtl C. Molecular study of a light-harvesting apoprotein of Giruudyopsis,stellifer(Chrysop hyceae ) [J]. Plant 1Vblecular Biology,1995,29：135一148.
30 Janson S，Creen B，Gossman A，et al. A proposal for ex- tending the nomenclature of light-harvesting proteins of the three transmembrane helix type [J].Plant 1Vblecular Biology Reporter,1999,17：221一224.
31 Leblanc C, Falciatore A, Watanabe M，et al. Semi-quantita- five RT PCR analysis of photoregulated gene expression in marine diatoms[J].Plant 1Vblecular Biology,1999,40:1031 一1044.

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