Cloning and Sequence Analysis of the Plasma Membrane Na+/H+Antiporter cDNA in Recretohalophyte Reaumuria trigyna Maxim.

doi:10.3969/j.issn.1000-7091.2013.03.001

Abstract

Abstract: Reaumuria trigyua Maxim. is an endangered small shrub with the features of a recretohalophyte. This species is endemic to the Eastern Alxa Western Ordos area and developed distinctive strategies to adapt to the semidesert and salty soil environment. A full}ength cDNA of the plasma Na⁺/H⁺antiporter (RtSOSl)was isolated from this species by using RT-CR and RACE technologies. The 3 829 by sequence comprised a 3 438 by open reading frame，encoding an 1 145 amino acids protein with the molecular weight of 126.76 kDa. Bioinformatics analyze reveals that RtSOSl composed of 11 transmembrane domains within its N terminal portion，and a hydrophilic cytoplasmic tail with the length approximately 700 amino acids in its Germinal portion. In the Germinal region，the phosphorylation domain and the auto-nhibited domain are found. The Homology comparison and phylogenetic analysis showed that RtSOSl is related to plasma membrane Na⁺/H⁺antiporter in other plant species.

Key words: Reaumuria trigyua Maxim, SOSl, cDNA cloning

CLC Number:

Dang Zhenhua, Zheng Linlin, Feng Zhi, Wang Yingchun. Cloning and Sequence Analysis of the Plasma Membrane Na⁺/H⁺Antiporter cDNA in Recretohalophyte Reaumuria trigyna Maxim.[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2013, 28(3): 1-6. doi: 10.3969/j.issn.1000-7091.2013.03.001.

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References

[1] Zhu .T K. Plant salt tolerance [J]. Trends in Plant Science,2001,6(2):66-71
[2] Shi H. The Arabidopsis thaliana salt tolerance gene SOSl encodes a putative Na⁺/H⁺antiporter [J]. Proceedings of the National Academy of Soiences , 2000 , 97(12):6896-6901.
[3] 马清，包爱科，伍国强，等质膜Na⁺/H⁺逆向转运蛋白与植物耐盐性[J].植物学报，2011, 46 (2):206-215.
[4] Oh D H , Ali Z , Hyeong C P ,et al. Consequences of SOSl defioiency:intracellular physiology and transcription[J] Plant Signal Behavior,2010,5(6):766-768.
[5] Oh D H ,Lee S Y, Bressan R A, et al. Intracellular consequenc;es of SOSl defioienc;y during salt stress [J] . Journal of Experimental Botany,2010,61 (4):1205-1213
[6] Wu Y,Ding N, Zhao X,et al. Molecular charac;terization of PeSOSl:the putative Na⁺/H⁺antiporter of Populu.s euphratica [J] . Plant Molecular Biology,2007 ,65(1):1-11
.
[7] Xu H , Jiang X,Zhan K , et al. Functional charac;terization of a wheat plasma membrane Na+/H+antiporter in yeast[J]. Archives of Biochemistry and Biophysic，2008，473(1):8-15.

[8] Mart nez 4tienza J, Jiang X ,Garoiadeblas B ,et al. Conservation of the salt overly sensitive pathway in rice[J]Plant Physiology,2007，143 (2):1001-1012.
[9] Ratner A , Jac;oby B. Effect of K+，its counter anion, and pH on sodium efflux from barley root tips [J] . Journal of Experimental Botany,1976 , 27 (5):843-852.
[10] Olias R, Eljakaoui Z, Li J, et al. The plasma membrane Na⁺/H⁺antiporter SOS1 is essential for salt tolerance in tomato and affects the partitioning of Na+between plant organs[J].Plant Cell&Environment 2009，32(7):904-916.
[11] Zhou G A ,Jiang Y ,Yang Q ,et al. Isolation and characterization of a new Na⁺/H⁺antiporter gene O.5WCAI from rice(Oryza .saliva L) [J] Mitochondrial DNA 2006,17(1):24-30.
[12] Wu C, Gao X , Kong X,et al. Molecular cloning and functional analysis of a Na+/H+antiporter gene ThNH 1from a halophytic plant Thellmagiella halophila [J] . Plant Molecular Biology Reporter,2009,27(1):1-12.
[13] Cosentino C,Fisc;her S E, Bertl A,et al. Na⁺/H⁺antiporters are differentially regulated in response to NaCI stress in leaves and roots of Me.sembrya}athemum cry.stalliaum [J] . New Phytologist, 2010 186(3):669-680.
[14] Yadav N S, Shukla P S ,Jha A,et al. The SbSOSl gene from the extreme halophyte Salicoraia brachiata enhances Na⁺loading in xylem and confers salt tolerance in transgenic tobacco [J] . BMC Plant Biology 2012 12:1RR.
[15] 王鹤，张高华，王旭达，等嶂茅耐盐基因sosl的克隆及序列分析[J].中国农业大学学报，2012, 17(3):28-33.
[16] 赵祥强玉米Na⁺/H⁺逆向转运蛋白基因ZmSOSI的克隆与鉴定田安徽农业科学，2009 ,37 (35):17843-17848.
[17] 马苏勇，祝建波，王爱英，等大叶补血草质膜Na⁺/H⁺逆向转运蛋白基因SOSI的克隆及对番茄的遗传转化日]生物技术通报，2010,36(9):1353-1358.
[18] 王澎，任晴雯，樊国盛黄瓜质膜型Na⁺/H⁺逆向转运蛋白基因(SOSI)的克隆与序列分析[J].上海交通大学学报:农业科学版，2012,30 (03):6-10.
[19] 玉祥星星草质膜型Na⁺/H⁺逆向转运蛋白基因的克隆和特性分析[J] 植物生理学通讯，2008,44(01):59-64
[20] Flowers T J, Colmer T D. Salinity tolerance in halophytes [J] . New Phytologist,2008，179 (4):945-963 Turkan I , Demiral T. Recent developments in understanding salinity tolerance [J] . Environmental and Experimental Botany,2009 ,67(1):2-9.
[22] 薛焱，王迎春，王同智濒危植物长叶红砂适应盐胁迫的生理生化机制研究田西北植物学报，2012,32(1):136-142.
[23] Dang Z H, Zheng L LWang J ,et ul. Transc riptomic profiling of the salty tress response in the wild receretohalophyte Reuumuriu trigyuu [J] . BMC Genomic 2013，14:29.
[24] 薛焱，王迎春.盐生植物长叶红砂泌盐特性的研究田中国沙漠,2008,28 (3):437-442.
[25] Shi H , Lee BWu S J，et ul. Overexpression of a plasma membrane Na⁺/H⁺antiporter gene improves salt tolerance in Arubidop.si,s thuliuuu [J] . Nature Biotechnology 2002,21(1):81-85.
[26] .
Quintero F J ,Martinez Atienza J,Villalta I ,et al. Activation of the plasma membrane Na+/H+antiporter Salt 0verly Sensitive 1 ( SOSl ) by phosphorylation of an autoinhibitory C terminal domain [J]. Proceedings of the National Academy of Soiences ,2011,108 (6):2611-2616.

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