Selecting of the Best Culture Condition of Poultry's Lactobacilli M8 and Optimizing the Culture Medium

doi:10.3321/j.issn:1000-7091.2007.02.042

Abstract

Abstract: In the experiment, we isolated 1 strain lactobacilli which was defined as Lactobacillus Pentosus (M8) from blind intestine of healthy Beijing You fowl. We got its growth curves and best culture condition in breezy and sealed bottles. The result showed: OD600nm reached to 1.011 and pH reached to 4.40 in breezy bottles. On the other hands, in the sealed bottles, OD600nm reached to 2.049 and pH reached to 3.42. Therefore, its optimum culture condition was in sealed bottles. We also got the growth OD600nm and pH in sealed bottles with different levels of inulin. The result was that the best percent of inulin was 3%, and the OD600nm reached to 2.937 and pH reached to 3.40.

Key words: Lactobacillus, Culture condition, Culture medium, Inulin

CLC Number:

ZHANG Dong-yan, JI Hai-feng, WANG Ya-min, HUANG Jian-guo, SHAN Da-cong. Selecting of the Best Culture Condition of Poultry's Lactobacilli M8 and Optimizing the Culture Medium[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2007, 22(2): 171-174. doi: 10.3321/j.issn:1000-7091.2007.02.042.

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