Detecting Chicken Infectious Anemia Virus with Polymerase Chain Reaction

doi:10.3321/j.issn:1000-7091.2003.03.025

Abstract

Abstract: A polymerase chain reaction (PCR) was constructed to detect chicken infectious anemia virus (CIAV). The assay used a pair of primers to amplify a 684 bp fragment of CIAV genome which located in the position 358 to 1 042. The amplified fragment was observed to detect infected cell culture and the sensitivity of the assay can be up to 1 fg. The result of amplification with NDV,MDV,IBDV, IBV,EDSV and E. Coli were negative. It showed that the assay was specific and highly sensitive. The positive fragments sere also be achieved in infected chicken tissues such as liver, spleen, thymus, bursa, kidney, white blood cell and rectal sub.

Key words: Chicken, Chicken infectious anemia, Virus, PCR

CLC Number:

S858.1

YANG Ring, XU Fu-zhou, WANG Jin-luo, CHEN Xiao-ling, MENG Yan-ni. Detecting Chicken Infectious Anemia Virus with Polymerase Chain Reaction[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2003, 18(3): 90-92. doi: 10.3321/j.issn:1000-7091.2003.03.025.

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http://www.hbnxb.net/EN/Y2003/V18/I3/90

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