Acta Agriculturae Boreali-Sinica

ACTA AGRICULTURAE BOREALI-SINICA ›› 2003, Vol. 18 ›› Issue (2): 14-16. doi: 10.3321/j.issn:1000-7091.2003.02.004

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Representational Difference Analysis of DNA Sequences Related to Cartap Resistance in Diamondback Moth,Plutella xylostella L.

CHENG Luo gen1, CHEN Zhi hao2, ZHANG Xiao fei1, LI Zhong ying2   


  1. 1. College of Life Science, Nanjing Normal University, Nanjing 210097,China;

    2. Institute of Plant Protection, Guizhou Academy of Agriculture Science, Guiyang 550006,China
  • Received:2002-07-17 Published:2003-06-28

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Abstract: The genomic difference between Plutella xylostella L.susceptible strain and cartap resistance near isogenic line strain was preliminarily analyzed by representationaLdifference analysis ( RDA)technique.In RDA system, the driver amplicon is susceptible strain while the tester amplicon is cartap resistance near isogenic line strain.After three rounds of subtractive hybridization, the different products were obtained and their sizes were about 150-300 bp.Comparisons between our sub-clone sequencing results and GenBank show that only one sub-clone sequence has P450 gene homology, other sub-clone sequences show no homology to knew gene.Preliminary judgment revealed that these segments might be new gene sequences.

Key words: :Diamondback moth, Plutella xylostella L , Cartap, Representational difference analysis( RDA)

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Cite this article

CHENG Luo gen, CHEN Zhi hao, ZHANG Xiao fei, LI Zhong ying. Representational Difference Analysis of DNA Sequences Related to Cartap Resistance in Diamondback Moth,Plutella xylostella L.[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2003, 18(2): 14-16. doi: 10.3321/j.issn:1000-7091.2003.02.004.

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