Abstract:
In this experiment five native female goats fitted with chronic rumen fistulas,T shaped duodenal cannulas were used to study the regulation of the ruminal nitrogen metabolism by injecting progesterone subcutandously(125mg/kg body weight),and to assay the ruminal bacteria progesterone receptor.The study showed that compared with the control period,the progesterone entered the rumen through blood and reached its peak 8 hours after the injection.Goat intake,duodenal chyme flow,as well as the microbe nitrogen(MCP N),UN and NH3 N in duodenal chyme,except for the total nitrogen,BPN and NAN,increased considerably(P<001).The radio receptor assay was used to analyse the progesterone receptor of goat rumen bacteria(BPR).The results showed that the maximal association content and Kd of BPR were 105.01±15.32 fmol/mg protein and 954±044×10 -8mol/L,respectively.It suggested that a certain specific progesterone receptor should exist in the ruminal bacteria.The results cited above showed that a series of bio effects could be stimulated by binding progesterone with BPR to obtain an increase both in the internal nitrogen and in MCP without affecting BPN.
Key words:
Progesterone,
Nitrogen metabolism of ruminal microorganisms,
Bacteriaprogesterone receptor
CLC Number:
Wang Jinluo, Chen Jie, Chen Weihua, Han Zhengkang. A Study of Regulating Nitrogen Metabolism of GoatRuminal Microorganisms with Progesterone[J]. ACTA AGRICULTURAE BOREALI-SINICA, 1998, 13(2): 127-131. doi: 10.3321/j.issn:1000-7091.1998.02.024.